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ETS1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS822058

MBS822058 is an antibody targeting ETS1. Also known as Anti-ETS1 (pT38) Phospho Antibody. Reactive in Human, Mouse, Rat, Bovine, Chicken, Dog, Pig, Rabbit. Host: Rabbit. Rabbit polyclonal antibody to ETS1 (pT38) More details

$235 / 0.03 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
ETS1 (pT38), Polyclonal Antibody
Full Product Name
Anti-ETS1 (pT38) Phospho Antibody
Product Gene Name
anti-ETS1 antibody
Product Synonym Names
EWSR2; Protein C-ets-1; p54
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Pig, Rabbit
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of ETS1 (pT38) protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
ETS1 (pT38) peptide (MBS823609) is used for blocking the activity of ETS1 (pT38) antibody (MBS822058)
OMIM
164720
3D Structure
ModBase 3D Structure for P14921
Entrez Gene
2113 (Human); 23871 (Mouse); 24356 (Rat)
SwissProt
P14921 (Human); P27577 (Mouse); P41156 (Rat)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ETS1. The exact sequence is proprietary.
Applications Tested/Suitable for anti-ETS1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-ETS1 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-ETS1 antibody

Western blot analysis of ETS1 (pT38) expression in LO2 (A), Jurkat (B), HepG2 (C), NIH3T3 (D), rat liver (E) whole cell lysates.
Rabbit Anti-ETS1 Polyclonal Antibody – Cat# MBS822058

Immunohistochemistry (IHC) of anti-ETS1 antibody

Immunohistochemical analysis of ETS1 (pT38) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-ETS1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS822058

Immunofluorescence (IF) of anti-ETS1 antibody

Immunofluorescent analysis of ETS1 (pT38) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-ETS1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS822058

Other Notes

Small volumes of anti-ETS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ETS1. It may not necessarily be applicable to this product.

NCBI GI #
219689118
NCBI GeneID
2113
NCBI Accession #
NP_001137292.1
UniProt Primary Accession #
P14921
UniProt Secondary Accession #
Q14278; Q16080; Q6N087; Q96AC5; A9UL17; F5GYX9
UniProt Related Accession #
P14921
GenBank Nucleotide #
NM_001143820.1
NCBI Official Full Name
protein C-ets-1 isoform 1
NCBI Official Synonym Full Names
v-ets avian erythroblastosis virus E26 oncogene homolog 1
NCBI Official Symbol
ETS1
NCBI Official Synonym Symbols
p54; ETS-1; EWSR2
UniProt Protein Name
Protein C-ets-1
UniProt Synonym Protein Names
p54
Protein Family
Protein
UniProt Gene Name
ETS1
UniProt Synonym Gene Names
EWSR2
UniProt Entry Name
ETS1_HUMAN
NCBI Protein Information
protein C-ets-1; v-ets avian erythroblastosis virus E2 oncogene homolog 1; Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1

NCBI Summary for ETS1

This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
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UniProt Comments for ETS1

Ets-1: a proto-oncogenic transcription factor homologous to the v-ets erythroblastosis virus oncogene. The DNA binding activity of Ets1 is controlled by kinases and transcription factors. It contributes to the regulation of cellular differentiation in hematopoietic cells. Ets1 promotes invasive behavior in endothelial cells, vascular smooth muscle cells and epithelial cancer cells. Regulates the expression of MMP1, MMP3, MMP9 and uPA as well as of VEGF and VEGFR gene expression. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Transcription factor; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: nucleoplasm; transcription factor complex; intercellular bridge; cytoplasm; nucleus

Molecular Function: histone acetyltransferase binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; hypothalamus development; positive regulation of erythrocyte differentiation; positive regulation of transcription, DNA-dependent; cell motility involved in cell locomotion; PML body organization and biogenesis; female pregnancy; positive regulation of cell motility; angiogenesis involved in wound healing; regulation of angiogenesis; response to estradiol stimulus; negative regulation of cell cycle; regulation of apoptosis; response to antibiotic; negative regulation of cell proliferation; positive regulation of angiogenesis; pituitary gland development; response to mechanical stimulus; positive regulation of cell proliferation; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; immune response
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Research Articles on ETS1

1. Synergy of aromatic residues and phosphoserines within the intrinsically disordered DNA-binding inhibitory elements of the Ets-1 transcription factor.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ETS1 antibody

 Reference Product  PubMed Publications
 JUN antibody  >128 publications with ETS1 and JUN
 FOS antibody  >28 publications with ETS1 and FOS
 MAPK1 antibody  >20 publications with ETS1 and MAPK1
 MMP1 antibody  >20 publications with ETS1 and MMP1
 MAPK3 antibody  >17 publications with ETS1 and MAPK3
 DAXX antibody  >9 publications with ETS1 and DAXX
 ANGPT2 antibody  >6 publications with ETS1 and ANGPT2
 FLT1 antibody  >2 publications with ETS1 and FLT1
 STAT6 antibody  >1 publications with ETS1 and STAT6

Pathways associated with anti-ETS1 antibody

 Products by Pathway  Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 HIF-2-alpha Transcription Factor Network Pathway antibodies  HIF-2-alpha Transcription Factor Network Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with anti-ETS1 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >137 publications with ETS1 and Carcinoma
 Neoplasm Invasiveness Antibodies  >75 publications with ETS1 and Neoplasm Invasiveness
 Neoplasm Metastasis Antibodies  >61 publications with ETS1 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >54 publications with ETS1 and Breast Neoplasms
 Inflammation Antibodies  >39 publications with ETS1 and Inflammation
 Cell Transformation, Neoplastic Antibodies  >32 publications with ETS1 and Cell Transformation, Neoplastic
 Disease Models, Animal Antibodies  >32 publications with ETS1 and Disease Models, Animal
 Kidney Diseases Antibodies  >26 publications with ETS1 and Kidney Diseases
 Carcinoma, Squamous Cell Antibodies  >26 publications with ETS1 and Carcinoma, Squamous Cell
 Intestinal Neoplasms Antibodies  >24 publications with ETS1 and Intestinal Neoplasms

Organs/Tissues associated with anti-ETS1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >231 publications with ETS1 and Blood
 Vascular Antibodies  >155 publications with ETS1 and Vascular
 Embryonic Tissue Antibodies  >63 publications with ETS1 and Embryonic Tissue
 Bone Antibodies  >55 publications with ETS1 and Bone
 Muscle Antibodies  >53 publications with ETS1 and Muscle
 Kidney Antibodies  >47 publications with ETS1 and Kidney
 Brain Antibodies  >44 publications with ETS1 and Brain
 Liver Antibodies  >43 publications with ETS1 and Liver
 Lung Antibodies  >39 publications with ETS1 and Lung
 Skin Antibodies  >36 publications with ETS1 and Skin
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