• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

EIF5B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS822149

MBS822149 is an antibody targeting EIF5B. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to EIF5B More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EIF5B, Polyclonal Antibody
Full Product Name
Anti-EIF5B Antibody
Product Gene Name
Product Synonym Names
IF2; KIAA0741; Eukaryotic translation initiation factor 5B; eIF-5B; Translation initiation factor IF-2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of EIF5B protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
EIF5B peptide (MBS824090) is used for blocking the activity of EIF5B antibody (MBS822149)
Molecular Weight
138,827 Da
OMIM
606086
3D Structure
ModBase 3D Structure for O60841
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EIF5B. The exact sequence is proprietary.
Applications Tested/Suitable for anti-EIF5B antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-EIF5B antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Western Blot (WB) of anti-EIF5B antibody

Western blot analysis of EIF5B expression in A549 (A), Jurkat (B) whole cell lysates.
Rabbit Anti-EIF5B Polyclonal Antibody – Cat# MBS822149

Immunohistochemistry (IHC) of anti-EIF5B antibody

Immunohistochemical analysis of EIF5B staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-EIF5B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS822149

Other Notes

Small volumes of anti-EIF5B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF5B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
eukaryotic translation initiation factor 5B
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 5B
NCBI Official Symbol
EIF5B
NCBI Official Synonym Symbols
IF2
UniProt Protein Name
Eukaryotic translation initiation factor 5B
UniProt Synonym Protein Names
Translation initiation factor IF-2
UniProt Gene Name
EIF5B
UniProt Synonym Gene Names
IF2; KIAA0741; eIF-5B
UniProt Entry Name
IF2P_HUMAN
NCBI Protein Information
eukaryotic translation initiation factor 5B; eIF-5B; translation initiation factor IF2; translation initiation factor IF-2

NCBI Summary for EIF5B

Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for EIF5B

EIF5B: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2. Belongs to the IF-2 family.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; translation initiation factor activity

Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; regulation of translational initiation
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EIF5B antibody

Diseases associated with anti-EIF5B antibody

Organs/Tissues associated with anti-EIF5B antibody

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000