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MMP8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS822204

MBS822204 is an antibody targeting MMP8. Reactive in Human, Rat. Host: Rabbit. Rabbit polyclonal antibody to MMP8 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MMP8, Polyclonal Antibody
Full Product Name
Anti-MMP8 Antibody
Product Gene Name
anti-MMP8 antibody
Product Synonym Names
CLG1; Neutrophil collagenase; Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of MMP8 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
MMP8 peptide (MBS823598) is used for blocking the activity of MMP8 antibody (MBS822204)
Molecular Weight
53,412 Da
OMIM
120355
3D Structure
ModBase 3D Structure for P22894
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MMP8. The exact sequence is proprietary.
Applications Tested/Suitable for anti-MMP8 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-MMP8 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-MMP8 antibody

Western blot analysis of MMP8 expression in SJRH30 (A), PMNL (B), Sw620 (C) whole cell lysates.
Rabbit Anti-MMP8 Polyclonal Antibody – Cat# MBS822204

Immunohistochemistry (IHC) of anti-MMP8 antibody

Immunohistochemical analysis of MMP8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-MMP8 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS822204

Immunofluorescence (IF) of anti-MMP8 antibody

Immunofluorescent analysis of MMP8 staining in SW620 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-MMP8 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS822204

Other Notes

Small volumes of anti-MMP8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP8. It may not necessarily be applicable to this product.

NCBI GI #
4505221
NCBI GeneID
4317
NCBI Accession #
NP_002415.1
UniProt Primary Accession #
P22894
UniProt Secondary Accession #
Q45F99
UniProt Related Accession #
P22894
GenBank Nucleotide #
NM_002424.2
NCBI Official Full Name
neutrophil collagenase preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 8 (neutrophil collagenase)
NCBI Official Symbol
MMP8
NCBI Official Synonym Symbols
HNC; CLG1; MMP-8; PMNL-CL
UniProt Protein Name
Neutrophil collagenase
UniProt Synonym Protein Names
Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL
Protein Family
Neutrophil collagenase
UniProt Gene Name
MMP8
UniProt Synonym Gene Names
CLG1; MMP-8; PMNL-CL
UniProt Entry Name
MMP8_HUMAN
NCBI Protein Information
neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase)

NCBI Summary for MMP8

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
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UniProt Comments for MMP8

MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.

Protein type: Secreted, signal peptide; EC 3.4.24.34; Protease; Secreted

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; serine-type endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; proteolysis
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Research Articles on MMP8

1. MMP8 rs1940475 SNP modifies the host response to inflammatory stimuli.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MMP8 antibody

 Reference Product  PubMed Publications
 TIMP1 antibody  >25 publications with MMP8 and TIMP1
 TIMP2 antibody  >11 publications with MMP8 and TIMP2
 CCL2 antibody  >6 publications with MMP8 and CCL2
 PTGS2 antibody  >2 publications with MMP8 and PTGS2
 EFNB1 antibody  >1 publications with MMP8 and EFNB1

Pathways associated with anti-MMP8 antibody

 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Collagen Degradation Pathway antibodies  Collagen Degradation Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Matrix Metalloproteinases Pathway antibodies  Matrix Metalloproteinases Pathway Diagram

Diseases associated with anti-MMP8 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >73 publications with MMP8 and Inflammation
 Cardiovascular Diseases Antibodies  >34 publications with MMP8 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >26 publications with MMP8 and Disease Models, Animal
 Fibrosis Antibodies  >26 publications with MMP8 and Fibrosis
 Lung Diseases Antibodies  >17 publications with MMP8 and Lung Diseases
 Hemorrhage Antibodies  >16 publications with MMP8 and Hemorrhage
 Atherosclerosis Antibodies  >14 publications with MMP8 and Atherosclerosis
 Necrosis Antibodies  >12 publications with MMP8 and Necrosis
 Wounds and Injuries Antibodies  >12 publications with MMP8 and Wounds and Injuries
 Heart Diseases Antibodies  >11 publications with MMP8 and Heart Diseases

Organs/Tissues associated with anti-MMP8 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >77 publications with MMP8 and Blood
 Connective Tissue Antibodies  >15 publications with MMP8 and Connective Tissue
 Skin Antibodies  >13 publications with MMP8 and Skin
 Liver Antibodies  >10 publications with MMP8 and Liver
 Bone Marrow Antibodies  >9 publications with MMP8 and Bone Marrow
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