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TRAF3IP3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS822211

MBS822211 is an antibody targeting TRAF3IP3. Reactive in Human, Bovine. Host: Rabbit. Rabbit polyclonal antibody to TRAF3IP3 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TRAF3IP3, Polyclonal Antibody
Full Product Name
Anti-TRAF3IP3 Antibody
Product Gene Name
Product Synonym Names
T3JAM; TRAF3-interacting JNK-activating modulator; TRAF3-interacting protein 3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Bovine
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of TRAF3IP3 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
TRAF3IP3 peptide (MBS823970) is used for blocking the activity of TRAF3IP3 antibody (MBS822211)
Molecular Weight
40,674 Da
OMIM
608255
3D Structure
ModBase 3D Structure for Q9Y228
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRAF3IP3. The exact sequence is proprietary.
Applications Tested/Suitable for anti-TRAF3IP3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-TRAF3IP3 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Western Blot (WB) of anti-TRAF3IP3 antibody

Western blot analysis of TRAF3IP3 expression in MDAMB453 (A), Jurkat (B), HeLa (C) whole cell lysates.
Rabbit Anti-TRAF3IP3 Polyclonal Antibody – Cat# MBS822211

Immunohistochemistry (IHC) of anti-TRAF3IP3 antibody

Immunohistochemical analysis of TRAF3IP3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-TRAF3IP3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS822211

Immunoprecipitation (IP) of anti-TRAF3IP3 antibody

Immunoprecipitation of TRAF3IP3 from 0.5mg Mouse cortex tissue lysate, using 5ug of anti-TRAF3IP3 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse cortex extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with anti-TRAF3IP3 Antibody.
Rabbit Anti-TRAF3IP3 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS822211

Other Notes

Small volumes of anti-TRAF3IP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TRAF3IP3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
TRAF3-interacting JNK-activating modulator isoform 2
NCBI Official Synonym Full Names
TRAF3 interacting protein 3
NCBI Official Symbol
TRAF3IP3
NCBI Official Synonym Symbols
T3JAM
UniProt Protein Name
TRAF3-interacting JNK-activating modulator
UniProt Synonym Protein Names
TRAF3-interacting protein 3
UniProt Gene Name
TRAF3IP3
UniProt Synonym Gene Names
T3JAM
UniProt Entry Name
T3JAM_HUMAN
NCBI Protein Information
TRAF3-interacting JNK-activating modulator; TRAF3 interacting Jun N terminal kinase (JNK) activating modulator

NCBI Summary for TRAF3IP3

The gene encodes a protein that mediates cell growth by modulating the c-Jun N-terminal kinase signal transduction pathway. The encoded protein may also interact with a large multi-protein assembly containing the phosphatase 2A catalytic subunit. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
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UniProt Comments for TRAF3IP3

TRAF3IP3: May function as an adapter molecule that regulates TRAF3-mediated JNK activation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: integral to membrane

Molecular Function: protein binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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