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SMAD6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS822217

MBS822217 is an antibody targeting SMAD6. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to SMAD6 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SMAD6, Polyclonal Antibody
Full Product Name
Anti-SMAD6 Antibody
Product Gene Name
anti-SMAD6 antibody
Product Synonym Names
MADH6; Mothers against decapentaplegic homolog 6; MAD homolog 6; Mothers against DPP homolog 6; SMAD family member 6; SMAD 6; Smad6; hSMAD6
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of SMAD6 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
SMAD6 peptide (MBS823736) is used for blocking the activity of SMAD6 antibody (MBS822217)
Molecular Weight
35,467 Da
OMIM
gene 614823
3D Structure
ModBase 3D Structure for O43541
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMAD6. The exact sequence is proprietary.
Applications Tested/Suitable for anti-SMAD6 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SMAD6 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200)

Western Blot (WB) of anti-SMAD6 antibody

Western blot analysis of SMAD6 expression in A204 (A), LN18 (B), L929 (C) whole cell lysates.
Rabbit Anti-SMAD6 Polyclonal Antibody – Cat# MBS822217

Immunohistochemistry (IHC) of anti-SMAD6 antibody

Immunohistochemical analysis of SMAD6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-SMAD6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS822217

Other Notes

Small volumes of anti-SMAD6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SMAD6. It may not necessarily be applicable to this product.

NCBI GI #
92859871
NCBI GeneID
4091
NCBI Accession #
NP_005576.3
UniProt Primary Accession #
O43541
UniProt Secondary Accession #
O43654; Q15799; Q7Z7L4; Q96E31; Q9UKZ3; A9J6M5
UniProt Related Accession #
O43541
GenBank Nucleotide #
NM_005585.4
NCBI Official Full Name
mothers against decapentaplegic homolog 6
NCBI Official Synonym Full Names
SMAD family member 6
NCBI Official Symbol
SMAD6
NCBI Official Synonym Symbols
AOVD2; MADH6; MADH7; HsT17432
UniProt Protein Name
Mothers against decapentaplegic homolog 6
UniProt Synonym Protein Names
SMAD family member 6; SMAD 6; Smad6; hSMAD6
Protein Family
Mothers against decapentaplegic
UniProt Gene Name
SMAD6
UniProt Synonym Gene Names
MADH6; MAD homolog 6; Mothers against DPP homolog 6; SMAD 6; Smad6; hSMAD6
UniProt Entry Name
SMAD6_HUMAN
NCBI Protein Information
mothers against decapentaplegic homolog 6; MAD homolog 6; SMAD, mothers against DPP homolog 6; mothers against decapentaplegic, drosophila, homolog of, 6

NCBI Summary for SMAD6

The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014]
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UniProt Comments for SMAD6

SMAD6: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions. Interacts with NEDD4L. Interacts with WWP1. Interacts with RNF111 and AXIN1. Interacts with TGF-beta type I receptor superfamily members, SMAD1, HOXC8 and HOXC9. Interacts with STAMBP and PRKX. Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue. Belongs to the dwarfin/SMAD family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: transcription factor complex; protein complex; cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; ubiquitin protein ligase binding; metal ion binding; chromatin binding; transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity; transcription factor activity

Biological Process: BMP signaling pathway; fat cell differentiation; negative regulation of cell proliferation; zygotic determination of dorsal/ventral axis; transcription, DNA-dependent; response to estrogen stimulus; ureteric bud development; transforming growth factor beta receptor signaling pathway; immune response; negative regulation of transforming growth factor beta receptor signaling pathway; cell-substrate adhesion; negative regulation of apoptosis; negative regulation of BMP signaling pathway

Disease: Aortic Valve Disease 2
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Research Articles on SMAD6

1. Single Nucleotide Polymorphisms in SMAD6 gene is associated with brain metastasis in non-small-cell lung cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMAD6 antibody

 Reference Product  PubMed Publications
 SMAD7 antibody  >172 publications with SMAD6 and SMAD7
 SMAD1 antibody  >125 publications with SMAD6 and SMAD1
 SMAD4 antibody  >89 publications with SMAD6 and SMAD4
 SMAD5 antibody  >56 publications with SMAD6 and SMAD5
 BMPR2 antibody  >27 publications with SMAD6 and BMPR2
 SMURF1 antibody  >15 publications with SMAD6 and SMURF1
 SMURF2 antibody  >7 publications with SMAD6 and SMURF2
 HOXC8 antibody  >2 publications with SMAD6 and HOXC8
 TAB1 antibody  >2 publications with SMAD6 and TAB1

Pathways associated with anti-SMAD6 antibody

 Products by Pathway  Pathway Diagram
 BMP Receptor Signaling Pathway antibodies  BMP Receptor Signaling Pathway Diagram
 BMP Signalling And Regulation Pathway antibodies  BMP Signalling And Regulation Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By BMP Pathway antibodies  Signaling By BMP Pathway Diagram
 TGF Beta Signaling Pathway antibodies  TGF Beta Signaling Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram

Diseases associated with anti-SMAD6 antibody

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >29 publications with SMAD6 and Fibrosis
 Lung Diseases Antibodies  >18 publications with SMAD6 and Lung Diseases
 Disease Models, Animal Antibodies  >15 publications with SMAD6 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >14 publications with SMAD6 and Cardiovascular Diseases
 Hypertension Antibodies  >13 publications with SMAD6 and Hypertension
 Inflammation Antibodies  >13 publications with SMAD6 and Inflammation
 Kidney Diseases Antibodies  >12 publications with SMAD6 and Kidney Diseases
 Pancreatic Neoplasms Antibodies  >8 publications with SMAD6 and Pancreatic Neoplasms
 Heart Diseases Antibodies  >8 publications with SMAD6 and Heart Diseases
 Necrosis Antibodies  >8 publications with SMAD6 and Necrosis

Organs/Tissues associated with anti-SMAD6 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >218 publications with SMAD6 and Bone
 Embryonic Tissue Antibodies  >32 publications with SMAD6 and Embryonic Tissue
 Kidney Antibodies  >23 publications with SMAD6 and Kidney
 Heart Antibodies  >21 publications with SMAD6 and Heart
 Lung Antibodies  >17 publications with SMAD6 and Lung
 Connective Tissue Antibodies  >16 publications with SMAD6 and Connective Tissue
 Brain Antibodies  >14 publications with SMAD6 and Brain
 Ovary Antibodies  >11 publications with SMAD6 and Ovary
 Parathyroid Antibodies  >8 publications with SMAD6 and Parathyroid
 Eye Antibodies  >7 publications with SMAD6 and Eye
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