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Cytokeratin 18 (KRT18) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS822236

MBS822236 is an antibody targeting KRT18. Also known as Anti-Cytokeratin 18 Antibody. Reactive in Human. Host: Rabbit. Rabbit polyclonal antibody to Cytokeratin 18 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 18 (KRT18), Polyclonal Antibody
Full Product Name
Anti-Cytokeratin 18 Antibody
Product Gene Name
anti-KRT18 antibody
Product Synonym Names
CYK18; Keratin, type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of Cytokeratin 18 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
Cytokeratin 18 peptide (MBS823613) is used for blocking the activity of Cytokeratin 18 antibody (MBS822236)
Molecular Weight
48,058 Da
OMIM
gene 215600
3D Structure
ModBase 3D Structure for P05783
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 18. The exact sequence is proprietary.
Applications Tested/Suitable for anti-KRT18 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-KRT18 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-KRT18 antibody

Western blot analysis of Cytokeratin 18 expression in HeLa (A), A431 (B), MCF7 (C) whole cell lysates.
Rabbit Anti-KRT18 Polyclonal Antibody – Cat# MBS822236

Immunohistochemistry (IHC) of anti-KRT18 antibody

Immunohistochemical analysis of Cytokeratin 18 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-KRT18 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS822236

Immunofluorescence (IF) of anti-KRT18 antibody

Immunofluorescent analysis of Cytokeratin 18 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-KRT18 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS822236

Other Notes

Small volumes of anti-KRT18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT18. It may not necessarily be applicable to this product.

NCBI GI #
4557888
NCBI GeneID
3875
NCBI Accession #
NP_000215.1
UniProt Primary Accession #
P05783
UniProt Secondary Accession #
Q53G38; Q5U0N8; Q9BW26
UniProt Related Accession #
P05783
GenBank Nucleotide #
NM_000224.2
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CYK18
UniProt Protein Name
Keratin, type I cytoskeletal 18
UniProt Synonym Protein Names
Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18
Protein Family
Keratin
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
UniProt Entry Name
K1C18_HUMAN
NCBI Protein Information
keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein

NCBI Summary for KRT18

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT18

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.

Protein type: Cytoskeletal; Nucleolus

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: perinuclear region of cytoplasm; cytoplasm; keratin filament; nucleolus; microtubule organizing center; intermediate filament

Molecular Function: protein binding; structural molecule activity

Biological Process: anatomical structure morphogenesis; viral reproduction; tumor necrosis factor-mediated signaling pathway; intermediate filament cytoskeleton organization and biogenesis; cell cycle; Golgi to plasma membrane CFTR protein transport; negative regulation of apoptosis

Disease: Cirrhosis, Familial
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Research Articles on KRT18

1. Median serum concentration of M30-CK18 was higher in chronic hepatitis C patients and was associated with inflammatory activity and liver fibrosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT18 antibody

 Reference Product  PubMed Publications
 KRT8 antibody  >48 publications with KRT18 and KRT8
 TCHP antibody  >1 publications with KRT18 and TCHP
 CASP6 antibody  >1 publications with KRT18 and CASP6

Pathways associated with anti-KRT18 antibody

 Products by Pathway  Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram

Diseases associated with anti-KRT18 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >31 publications with KRT18 and Liver Diseases
 Carcinoma Antibodies  >30 publications with KRT18 and Carcinoma
 Adenocarcinoma Antibodies  >15 publications with KRT18 and Adenocarcinoma
 Breast Neoplasms Antibodies  >15 publications with KRT18 and Breast Neoplasms
 Fibrosis Antibodies  >13 publications with KRT18 and Fibrosis
 Neoplasm Metastasis Antibodies  >11 publications with KRT18 and Neoplasm Metastasis
 Fatty Liver Antibodies  >11 publications with KRT18 and Fatty Liver
 Necrosis Antibodies  >9 publications with KRT18 and Necrosis
 Liver Cirrhosis Antibodies  >8 publications with KRT18 and Liver Cirrhosis
 Liver Neoplasms Antibodies  >7 publications with KRT18 and Liver Neoplasms

Organs/Tissues associated with anti-KRT18 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >41 publications with KRT18 and Liver
 Blood Antibodies  >24 publications with KRT18 and Blood
 Prostate Antibodies  >8 publications with KRT18 and Prostate
 Lymph Antibodies  >8 publications with KRT18 and Lymph
 Lymph Node Antibodies  >8 publications with KRT18 and Lymph Node
 Kidney Antibodies  >7 publications with KRT18 and Kidney
 Eye Antibodies  >6 publications with KRT18 and Eye
 Muscle Antibodies  >6 publications with KRT18 and Muscle
 Stomach Antibodies  >5 publications with KRT18 and Stomach
 Lung Antibodies  >4 publications with KRT18 and Lung
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