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P130 Cas (Py249) (BCAR1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS822260

MBS822260 is an antibody targeting BCAR1. Also known as Anti-p130 Cas (pY249) Phospho Antibody. Reactive in Human, Mouse, Rat, Bovine. Host: Rabbit. Rabbit polyclonal antibody to p130 Cas (pY249) More details

$235 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
p130 Cas (pY249) (BCAR1), Polyclonal Antibody
Full Product Name
Anti-p130 Cas (pY249) Phospho Antibody
Product Gene Name
anti-BCAR1 antibody
Product Synonym Names
CAS; CASS1; CRKAS; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of p130 Cas (pY249) protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
p130 Cas (pY249) peptide (MBS822632) is used for blocking the activity of p130 Cas (pY249) antibody (MBS822260)
Molecular Weight
93,291 Da
OMIM
602941
3D Structure
ModBase 3D Structure for P56945
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p130 Cas. The exact sequence is proprietary.
Applications Tested/Suitable for anti-BCAR1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)
Application Notes for anti-BCAR1 antibody
WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-BCAR1 antibody

Western blot analysis of p130 Cas (pY249) expression in HEK293T EGF-treated (A), NIH3T3 EGF-treated (B), Raw264.7 EGF-treated (C), PC12 EGF-treated (D) whole cell lysates.
Rabbit Anti-BCAR1 Polyclonal Antibody – Cat# MBS822260

Immunofluorescence (IF) of anti-BCAR1 antibody

Immunofluorescent analysis of p130 Cas (pY249) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-BCAR1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS822260

Other Notes

Small volumes of anti-BCAR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BCAR1. It may not necessarily be applicable to this product.

NCBI GI #
282398112
NCBI GeneID
9564
NCBI Accession #
NP_001164185.1
UniProt Primary Accession #
P56945
UniProt Secondary Accession #
B3KWD7; B4DEV4; B4DGB5; B4DIW5; B7Z7X7; E9PCL5; E9PCV2; F5GXA2; F5GXV6; F5H7Z0; F8WA69
UniProt Related Accession #
P56945
GenBank Nucleotide #
NM_001170714.1
NCBI Official Full Name
breast cancer anti-estrogen resistance protein 1 isoform 1
NCBI Official Synonym Full Names
breast cancer anti-estrogen resistance 1
NCBI Official Symbol
BCAR1
NCBI Official Synonym Symbols
CAS; CAS1; CASS1; CRKAS; P130Cas
UniProt Protein Name
Breast cancer anti-estrogen resistance protein 1
UniProt Synonym Protein Names
CRK-associated substrate; Cas scaffolding protein family member 1; p130cas
Protein Family
Breast cancer anti-estrogen resistance protein
UniProt Gene Name
BCAR1
UniProt Synonym Gene Names
CAS; CASS1; CRKAS
UniProt Entry Name
BCAR1_HUMAN
NCBI Protein Information
breast cancer anti-estrogen resistance protein 1; Crk-associated substrate p130Cas; Cas scaffolding protein family member 1

NCBI Summary for BCAR1

BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009]

UniProt Comments for BCAR1

P130Cas: a docking protein containing multiple protein-protein interaction domains. Plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. The amino-terminal SH3 domain regulates its interaction with focal adhesion kinase (FAK) and the FAK-related kinase PYK2 and also with tyrosine phosphatases PTP-1B and PTP-PEST. Overexpression confers antiestrogen resistance on breast cancer cells.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: ruffle; focal adhesion; lamellipodium; cytoplasm; nucleolus; plasma membrane; cytosol; actin cytoskeleton

Molecular Function: signal transducer activity; protein binding; protein kinase binding; SH3 domain binding

Biological Process: antigen receptor-mediated signaling pathway; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; cell migration; nerve growth factor receptor signaling pathway; actin filament organization; hepatocyte growth factor receptor signaling pathway; platelet-derived growth factor receptor signaling pathway; T cell receptor signaling pathway; regulation of apoptosis; cell proliferation; G-protein coupled receptor protein signaling pathway; B cell receptor signaling pathway; cell division; insulin receptor signaling pathway; regulation of cell growth; cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood coagulation; positive regulation of cell migration
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Research Articles on BCAR1

1. Our results show that endogenous Cul5 suppresses epithelial cell transformation by several pathways, including inhibition of Src-Cas-induced ruffling through SOCS6

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BCAR1 antibody

 Reference Product  PubMed Publications
 CRK antibody  >391 publications with BCAR1 and CRK
 SRC antibody  >166 publications with BCAR1 and SRC
 PTK2 antibody  >105 publications with BCAR1 and PTK2
 PXN antibody  >69 publications with BCAR1 and PXN
 FYN antibody  >13 publications with BCAR1 and FYN
 PTK2B antibody  >12 publications with BCAR1 and PTK2B
 CRKL antibody  >9 publications with BCAR1 and CRKL
 PTPN12 antibody  >9 publications with BCAR1 and PTPN12
 PTPN1 antibody  >4 publications with BCAR1 and PTPN1

Pathways associated with anti-BCAR1 antibody

 Products by Pathway  Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 EPHA2 Forward Signaling Pathway antibodies  EPHA2 Forward Signaling Pathway Diagram

Diseases associated with anti-BCAR1 antibody

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >34 publications with BCAR1 and Cell Transformation, Neoplastic
 Neoplasm Invasiveness Antibodies  >27 publications with BCAR1 and Neoplasm Invasiveness
 Neoplasm Metastasis Antibodies  >18 publications with BCAR1 and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >10 publications with BCAR1 and Disease Models, Animal
 Inflammation Antibodies  >6 publications with BCAR1 and Inflammation
 Cardiovascular Diseases Antibodies  >6 publications with BCAR1 and Cardiovascular Diseases
 Hypertrophy Antibodies  >4 publications with BCAR1 and Hypertrophy
 Hypertension Antibodies  >4 publications with BCAR1 and Hypertension
 Kidney Diseases Antibodies  >3 publications with BCAR1 and Kidney Diseases
 Liver Neoplasms Antibodies  >3 publications with BCAR1 and Liver Neoplasms

Organs/Tissues associated with anti-BCAR1 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >40 publications with BCAR1 and Vascular
 Muscle Antibodies  >23 publications with BCAR1 and Muscle
 Lung Antibodies  >22 publications with BCAR1 and Lung
 Bone Antibodies  >18 publications with BCAR1 and Bone
 Heart Antibodies  >12 publications with BCAR1 and Heart
 Kidney Antibodies  >12 publications with BCAR1 and Kidney
 Brain Antibodies  >12 publications with BCAR1 and Brain
 Prostate Antibodies  >11 publications with BCAR1 and Prostate
 Pancreas Antibodies  >8 publications with BCAR1 and Pancreas
 Liver Antibodies  >6 publications with BCAR1 and Liver
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