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BRF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS822292

MBS822292 is an antibody targeting BRF1. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to BRF1 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BRF1, Polyclonal Antibody
Full Product Name
Anti-BRF1 Antibody
Product Gene Name
anti-BRF1 antibody
Product Synonym Names
BRF; GTF3B; TAF3B2; TAF3C; Transcription factor IIIB 90 kDa subunit; TFIIIB90; hTFIIIB90; B-related factor 1; BRF-1; hBRF; TAF3B2; TATA box-binding protein-associated factor, RNA polymerase III, subunit 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of BRF1 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
BRF1 peptide (MBS824379) is used for blocking the activity of BRF1 antibody (MBS822292)
Molecular Weight
47,978 Da
OMIM
604902
3D Structure
ModBase 3D Structure for Q92994
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BRF1. The exact sequence is proprietary.
Applications Tested/Suitable for anti-BRF1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-BRF1 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-BRF1 antibody

Western blot analysis of BRF1 expression in Jurkat (A), NIH3T3 (B) whole cell lysates.
Rabbit Anti-BRF1 Polyclonal Antibody – Cat# MBS822292

Immunohistochemistry (IHC) of anti-BRF1 antibody

Immunohistochemical analysis of BRF1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-BRF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS822292

Immunofluorescence (IF) of anti-BRF1 antibody

Immunofluorescent analysis of BRF1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-BRF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS822292

Other Notes

Small volumes of anti-BRF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BRF1. It may not necessarily be applicable to this product.

NCBI GI #
338753402
NCBI GeneID
2972
NCBI Accession #
NP_001229715.1
UniProt Primary Accession #
Q92994
UniProt Secondary Accession #
Q13223; Q3SYD9; Q5PR24; Q6IQ02; Q96KX3; Q9HCW6; B3KU36; B4DIG5; B7Z2N3; F5H5Z7; F8WA46
UniProt Related Accession #
Q92994
GenBank Nucleotide #
NM_001242786.1
NCBI Official Full Name
transcription factor IIIB 90 kDa subunit isoform 4
NCBI Official Synonym Full Names
BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
NCBI Official Symbol
BRF1
NCBI Official Synonym Symbols
BRF; hBRF; BRF-1; GTF3B; TAF3C; TAF3B2; TF3B90; TAFIII90; TFIIIB90; HEL-S-76p
UniProt Protein Name
Transcription factor IIIB 90 kDa subunit
UniProt Synonym Protein Names
B-related factor 1; BRF-1; hBRF; TAF3B2; TATA box-binding protein-associated factor, RNA polymerase III, subunit 2
Protein Family
Transcription factor
UniProt Gene Name
BRF1
UniProt Synonym Gene Names
BRF; GTF3B; TAF3B2; TAF3C; TFIIIB90; hTFIIIB90; BRF-1; hBRF
UniProt Entry Name
TF3B_HUMAN
NCBI Protein Information
transcription factor IIIB 90 kDa subunit; B - related factor 1; general transcription factor IIIB, 90kD subunit; TBP - associated factor, RNA polymerase III, 90kD; epididymis secretory sperm binding protein Li 76p; TATA box binding protein (TBP)-associated factor 3C; BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB; TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3B subunit 2

NCBI Summary for BRF1

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011]
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UniProt Comments for BRF1

GTF3B: General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter. Belongs to the TFIIB family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 14q

Cellular Component: nucleoplasm; transcription factor TFIIIB complex

Molecular Function: zinc ion binding; TATA-binding protein binding

Biological Process: transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase III promoter; tRNA transcription; regulation of transcription, DNA-dependent; gene expression; regulation of mRNA stability; rRNA transcription

Disease: Cerebellofaciodental Syndrome
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Research Articles on BRF1

1. these observations are in favor of a cell- and context-dependent regulation of Tis11b by hypoxia, which then contributes to modulation of angiogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BRF1 antibody

 Reference Product  PubMed Publications
 TBP antibody  >55 publications with BRF1 and TBP
 BDP1 antibody  >25 publications with BRF1 and BDP1
 HMGB2 antibody  >1 publications with BRF1 and HMGB2

Pathways associated with anti-BRF1 antibody

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 RNA Polymerase III Abortive And Retractive Initiation Pathway antibodies  RNA Polymerase III Abortive And Retractive Initiation Pathway Diagram
 RNA Polymerase III Transcription Pathway antibodies  RNA Polymerase III Transcription Pathway Diagram
 RNA Polymerase III Transcription Initiation Pathway antibodies  RNA Polymerase III Transcription Initiation Pathway Diagram
 RNA Polymerase III Transcription Initiation From Type 1 Promoter Pathway antibodies  RNA Polymerase III Transcription Initiation From Type 1 Promoter Pathway Diagram
 RNA Polymerase III Transcription Initiation From Type 2 Promoter Pathway antibodies  RNA Polymerase III Transcription Initiation From Type 2 Promoter Pathway Diagram

Diseases associated with anti-BRF1 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >5 publications with BRF1 and Breast Neoplasms
 Necrosis Antibodies  >3 publications with BRF1 and Necrosis
 Inflammation Antibodies  >2 publications with BRF1 and Inflammation
 Carcinoma, Hepatocellular Antibodies  >2 publications with BRF1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >2 publications with BRF1 and Liver Neoplasms
 Liver Diseases Antibodies  >2 publications with BRF1 and Liver Diseases
 Hypertrophy Antibodies  >1 publications with BRF1 and Hypertrophy
 Fatty Liver Antibodies  >1 publications with BRF1 and Fatty Liver
 Kidney Diseases Antibodies  >1 publications with BRF1 and Kidney Diseases
 Precancerous Conditions Antibodies  >1 publications with BRF1 and Precancerous Conditions

Organs/Tissues associated with anti-BRF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >5 publications with BRF1 and Heart
 Liver Antibodies  >3 publications with BRF1 and Liver
 Muscle Antibodies  >3 publications with BRF1 and Muscle
 Prostate Antibodies  >2 publications with BRF1 and Prostate
 Vascular Antibodies  >2 publications with BRF1 and Vascular
 Bone Antibodies  >2 publications with BRF1 and Bone
 Blood Antibodies  >2 publications with BRF1 and Blood
 Embryonic Tissue Antibodies  >1 publications with BRF1 and Embryonic Tissue
 Cervix Antibodies  >1 publications with BRF1 and Cervix
 Uterus Antibodies  >1 publications with BRF1 and Uterus
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