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Negative Control RNA Control

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8241404

MBS8241404 is an RNA control targeting Negative Control. Also known as siRNA Negative Control. Negative Control for experiments using targeted siRNA transfection More details

$190 / 2.5 nmol +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Negative Control, RNA Control
Full Product Name
siRNA Negative Control
Form/Format
Lyophilized powder
Specificity
Negative Control Duplexes are ideal for use in RNA interference (RNAi) experiments as a control for sequence independent effects following siRNA delivery in any vertebrate cell line.
Preparation and Storage
Store at -20 degree C for one year.
Source
Synthetic
Direction for Use
Lab recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water.
Components
siRNA Negative Control is a non-targeting 21 nt siRNA designed.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Applications Tested/Suitable for Negative Control rna control
RNA Interference (RNAi)
Application Notes for Negative Control rna control
It is recommended as a negative control for evaluating RNAi off-target effects, and in order to verify the accuracy of gene specific siRNA dependent RNAi.

Other Notes

Small volumes of Negative Control rna control vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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