Kallikrein 5 (KLK5) ELISA Kit for Human

Background: Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.

Principle of the Assay: The MyBioSource' Human Kallikrein 5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Kallikrein 5 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Kallikrein 5 coated on a 96-well plate. Standards and samples are pipetted into the wells and Kallikrein 5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Kallikrein 5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Kallikrein 5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Small volumes of KLK5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS824564 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kallikrein 5 (KLK5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KLK5. The ELISA analytical biochemical technique of the MBS824564 kit is based on KLK5 antibody-KLK5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KLK5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KLK5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).