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Tpa (PLAT) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS824607

Background: Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type… More details

$640 / 96 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
tPA (PLAT), ELISA Kit
Full Product Name
Human tPA ELISA Kit
Product Gene Name
PLAT elisa kit
Product Synonym Names
Tissue-type plasminogen activator; t-PA; t-plasminogen activator; tPA; Alteplase; Reteplase
Species Reactivity
Human
Samples
Culture Supernatants, Serum, Plasma, Tissue Homogenates
Sensitivity
< 10 pg/ml
Specificity
The Human tPA ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human tPA proteins within the range of 31.2 pg/ml - 2000 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Molecular Weight
44,373 Da
OMIM
173370
3D Structure
ModBase 3D Structure for P00750
Detection Method
Colorimetric
Applications Tested/Suitable for PLAT elisa kit
Sandwich ELISA (SE)

Typical Testing Data/Standard Curve (for reference only) of PLAT elisa kit

Human PLAT ELISA Kit – Cat# MBS824607

Other Notes

Small volumes of PLAT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLAT purchase

MBS824607 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tPA (PLAT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLAT. The ELISA analytical biochemical technique of the MBS824607 kit is based on PLAT antibody-PLAT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLAT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLAT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PLAT. It may not necessarily be applicable to this product.

NCBI GI #
4505861
NCBI GeneID
5327
NCBI Accession #
NP_000921.1
UniProt Primary Accession #
P00750
UniProt Secondary Accession #
Q15103; Q503B0; Q6PJA5; Q7Z7N2; Q86YK8; Q9BU99; Q9BZW1; A8K022; B2R8E8
UniProt Related Accession #
P00750
GenBank Nucleotide #
NM_000930.3
NCBI Official Full Name
tissue-type plasminogen activator isoform 1 preproprotein
NCBI Official Synonym Full Names
plasminogen activator, tissue
NCBI Official Symbol
PLAT
NCBI Official Synonym Symbols
TPA; T-PA
UniProt Protein Name
Tissue-type plasminogen activator
UniProt Synonym Protein Names
INN: Alteplase; INN: ReteplaseCleaved into the following 2 chains:Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B
Protein Family
Tissue-type plasminogen activator
UniProt Gene Name
PLAT
UniProt Synonym Gene Names
t-PA; t-plasminogen activator; tPA
UniProt Entry Name
TPA_HUMAN
NCBI Protein Information
tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA)

NCBI Summary for PLAT

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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UniProt Comments for PLAT

PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Protease; Secreted; Cytoskeletal; Motility/polarity/chemotaxis; EC 3.4.21.68; Vesicle

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: extracellular matrix; extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: negative regulation of proteolysis; synaptic transmission, glutamatergic; response to peptide hormone stimulus; response to cAMP; smooth muscle cell migration; regulation of synaptic plasticity; protein modification process; platelet-derived growth factor receptor signaling pathway; proteolysis; fibrinolysis; response to hypoxia; blood coagulation; transmembrane transport; plasminogen activation

Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator
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Research Articles on PLAT

1. plasminogen and plasmin but not tissue-type plasminogen activator have roles in regulating fibrinolysis by C-terminal lysines

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PLAT elisa kit

 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Dissolution Of Fibrin Clot Pathway antibodies  Dissolution Of Fibrin Clot Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Selenium Pathway antibodies  Selenium Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram

Diseases associated with PLAT elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >37 publications with PLAT and Inflammation
 Arterial Occlusive Diseases Antibodies  >25 publications with PLAT and Arterial Occlusive Diseases
 Atherosclerosis Antibodies  >25 publications with PLAT and Atherosclerosis
 Thrombosis Antibodies  >19 publications with PLAT and Thrombosis
 Necrosis Antibodies  >17 publications with PLAT and Necrosis
 Cerebral Infarction Antibodies  >15 publications with PLAT and Cerebral Infarction
 Myocardial Infarction Antibodies  >14 publications with PLAT and Myocardial Infarction
 Hypertension Antibodies  >14 publications with PLAT and Hypertension
 Stroke Antibodies  >14 publications with PLAT and Stroke
 Embolism and Thrombosis Antibodies  >10 publications with PLAT and Embolism and Thrombosis

Organs/Tissues associated with PLAT elisa kit

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >54 publications with PLAT and Vascular
 Liver Antibodies  >49 publications with PLAT and Liver
 Brain Antibodies  >42 publications with PLAT and Brain
 Heart Antibodies  >40 publications with PLAT and Heart
 Lung Antibodies  >34 publications with PLAT and Lung
 Muscle Antibodies  >28 publications with PLAT and Muscle
 Bone Antibodies  >20 publications with PLAT and Bone
 Intestine Antibodies  >17 publications with PLAT and Intestine
 Embryonic Tissue Antibodies  >16 publications with PLAT and Embryonic Tissue
 Kidney Antibodies  >15 publications with PLAT and Kidney
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