CXCL10 ELISA Kit for Human

Principle of the Assay: The MyBioSource' Human CXCL10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL10 in Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Human CXCL10 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.

Small volumes of CXCL10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS824608 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CXCL10, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CXCL10. The ELISA analytical biochemical technique of the MBS824608 kit is based on CXCL10 antibody-CXCL10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CXCL10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CXCL10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).