CCL24 ELISA Kit for Mouse

Principle of the Assay: The MyBioSource' Mouse CCL24 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CCL24 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CCL24 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL24 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CCL24 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL24 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Chemokine (C-C motif) ligand 24 (CCL24) also known as myeloid progenitor inhibitory factor 2 (MPIF-2) or eosinophil chemotactic protein 2 (eotaxin-2) is a protein that in humans is encoded by the CCL24 gene. By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23. CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils. This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.

Small volumes of CCL24 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS824712 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CCL24, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CCL24. The ELISA analytical biochemical technique of the MBS824712 kit is based on CCL24 antibody-CCL24 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCL24 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCL24. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).