CXCL13 ELISA Kit for Human

Principle of the Assay: The MyBioSource' Human CXCL13 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL13 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CXCL13 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the Tcell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells.

Small volumes of CXCL13 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS824727 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CXCL13, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CXCL13. The ELISA analytical biochemical technique of the MBS824727 kit is based on CXCL13 antibody-CXCL13 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CXCL13 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CXCL13. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).