CCL6 ELISA Kit for Mouse

Principle of the Assay: The MyBioSource' Mouse CCL6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CCL6 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CCL6 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CCL6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.!!Background/Introduction: Chemokine (C-C motif) ligand 6 (CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents.In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin (e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

Small volumes of CCL6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS824742 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CCL6, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CCL6. The ELISA analytical biochemical technique of the MBS824742 kit is based on CCL6 antibody-CCL6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCL6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCL6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).