Tetranectin (CLEC3B) ELISA Kit for Human

Principle of the Assay: The MyBioSource' Human Tetranectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Tetranectin in Cell Culture Supernatants, Serum, Tissue Homogenates. This assay employs an antibody specific for Human Tetranectin coated on a 96-well plate. Standards and samples are pipetted into the wells and Tetranectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Tetranectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Tetranectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.!!Background/Introduction: Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and noncovalently bound polypeptide chains, each of 181 amino acid residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.

Small volumes of CLEC3B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS824758 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tetranectin (CLEC3B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CLEC3B. The ELISA analytical biochemical technique of the MBS824758 kit is based on CLEC3B antibody-CLEC3B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLEC3B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLEC3B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).