SPINK1 ELISA Kit for Human

Background: Serine protease inhibitor Kazal-type 1, also called TATI or PSTI, is a protein that in humans is encoded by the SPINK1 gene. It is mapped to 5q32. SPINK1 gene contains 4 exons spanning approximately 7.5 kb. It was initially detected in urine of patients with ovarian cancer. This peptide is also produced by the mucosa of the gastrointestinal tract where it may protect the mucosal cells from proteolytic breakdown. The SPINK1 gene encodes pancreatic secretory trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. Its physiologic role was thought to be the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.

Principle of the Assay: The Human SPINK1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SPINK1 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human SPINK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and SPINK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SPINK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SPINK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Small volumes of SPINK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS824791 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the SPINK1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SPINK1. The ELISA analytical biochemical technique of the MBS824791 kit is based on SPINK1 antibody-SPINK1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPINK1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPINK1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).