Nidogen 1 (NID1) ELISA Kit for Human

Background: Nidogen-1 (NID-1), also known as Entactin, is a protein that in humans is encoded by the NID1 gene. It is a member of the nidogen family of basement membrane glycoproteins. This gene is mapped to 1q42.3. Nidogen-1 is a component of the basement membrane alongside other components such as collagen type IV, proteoglycans (heparan sulfate and glycosaminoglycans), laminin and fibronectin. The protein interacts with several other components of basement membranes. Structurally it (along with perlecan) connects the networks formed by collagens and laminins to each other. It may also play a role in cell interactions with the extracellular matrix. Nidogen-1 also can serve as a bridge between the 2 most abundant molecules in the basement membrane: type IV collagen and laminin.

Principle of the Assay: The Human Nidogen 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Nidogen 1 in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Human Nidogen 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Nidogen 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Nidogen 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Nidogen 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Small volumes of NID1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS824834 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nidogen 1 (NID1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NID1. The ELISA analytical biochemical technique of the MBS824834 kit is based on NID1 antibody-NID1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NID1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NID1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).