TIMP1 ELISA Kit for Human

Name: Human TIMP1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS824841
Price: 615 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS824841

Sandwich ELISA for quantitative detection of Human TIMP1 concentrations. More details

$615 / 96 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

TIMP1, ELISA Kit

Full Product Name

Human TIMP1 ELISA Kit

Product Gene Name

TIMP1 elisa kit

Product Synonym Names

CLGI; TIMP; Metalloproteinase inhibitor 1; Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; TIMP-1

Species Reactivity

Human

Assay Type

Sandwich

Samples

Cell Culture Supernatants, Serum, Plasma, Saliva

Detection Range

156 pg/ml - 10000 pg/ml

Sensitivity

5 pg/ml

Preparation and Storage

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Molecular Weight

23,171 Da

OMIM

305370

3D Structure

ModBase 3D Structure for P01033

Detection Method

Colorimetric

Applications Tested/Suitable for TIMP1 elisa kit

Sandwich ELISA (SE)

Other Notes

Small volumes of TIMP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIMP1 purchase

MBS824841 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TIMP1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TIMP1. The ELISA analytical biochemical technique of the MBS824841 kit is based on TIMP1 antibody-TIMP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TIMP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TIMP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TIMP1. It may not necessarily be applicable to this product.

NCBI GI #

4507509

NCBI GeneID

7076

NCBI Accession #

NP_003245.1

UniProt Primary Accession #

P01033

UniProt Secondary Accession #

Q14252; Q9UCU1

UniProt Related Accession #

P01033

GenBank Nucleotide #

NM_003254.2

NCBI Official Full Name

metalloproteinase inhibitor 1

NCBI Official Synonym Full Names

TIMP metallopeptidase inhibitor 1

NCBI Official Symbol

TIMP1

NCBI Official Synonym Symbols

EPA; EPO; HCI; CLGI; TIMP

UniProt Protein Name

Metalloproteinase inhibitor 1

UniProt Synonym Protein Names

Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; TIMP-1

Protein Family

Metalloproteinase inhibitor

UniProt Gene Name

TIMP1

UniProt Synonym Gene Names

CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1

UniProt Entry Name

TIMP1_HUMAN

NCBI Protein Information

metalloproteinase inhibitor 1; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1

NCBI Summary for TIMP1

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]

UniProt Comments for TIMP1

TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xp11.3-p11.23

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; growth factor activity; protease binding; metal ion binding; cytokine activity

Biological Process: platelet activation; response to peptide hormone stimulus; extracellular matrix organization and biogenesis; negative regulation of metalloenzyme activity; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; blood coagulation; aging; negative regulation of apoptosis

Research Articles on TIMP1

1. These data suggest a pivotal role of the anti-angiogenic factor TIMP-1 in intercellular tumor-endothelial cell communication resulting in anti-angiogenic features of endothelial cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TIMP1 elisa kit

Reference Product	PubMed Publications
MMP9 elisa kit	>289 publications with TIMP1 and MMP9
MMP2 elisa kit	>259 publications with TIMP1 and MMP2
MMP1 elisa kit	>142 publications with TIMP1 and MMP1
MMP3 elisa kit	>78 publications with TIMP1 and MMP3
TGFB1 elisa kit	>75 publications with TIMP1 and TGFB1
MMP14 elisa kit	>26 publications with TIMP1 and MMP14
SERPINE1 elisa kit	>20 publications with TIMP1 and SERPINE1
FN1 elisa kit	>10 publications with TIMP1 and FN1
EGF elisa kit	>8 publications with TIMP1 and EGF

Pathways associated with TIMP1 elisa kit

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
HIF-1 Signaling Pathway antibodies	HIF-1 Signaling Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
IL6-mediated Signaling Events Pathway antibodies	IL6-mediated Signaling Events Pathway Diagram
Matrix Metalloproteinases Pathway antibodies	Matrix Metalloproteinases Pathway Diagram
Platelet Activation, Signaling And Aggregation Pathway antibodies	Platelet Activation, Signaling And Aggregation Pathway Diagram
Platelet Degranulation Pathway antibodies	Platelet Degranulation Pathway Diagram
Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies	Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram

Diseases associated with TIMP1 elisa kit

Disease Name	Pubmed Publications
Fibrosis Antibodies	>162 publications with TIMP1 and Fibrosis
Inflammation Antibodies	>144 publications with TIMP1 and Inflammation
Cardiovascular Diseases Antibodies	>139 publications with TIMP1 and Cardiovascular Diseases
Vascular Diseases Antibodies	>103 publications with TIMP1 and Vascular Diseases
Liver Diseases Antibodies	>83 publications with TIMP1 and Liver Diseases
Necrosis Antibodies	>79 publications with TIMP1 and Necrosis
Heart Diseases Antibodies	>73 publications with TIMP1 and Heart Diseases
Female Urogenital Diseases Antibodies	>58 publications with TIMP1 and Female Urogenital Diseases
Liver Cirrhosis Antibodies	>54 publications with TIMP1 and Liver Cirrhosis
Breast Neoplasms Antibodies	>38 publications with TIMP1 and Breast Neoplasms

Organs/Tissues associated with TIMP1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>267 publications with TIMP1 and Blood
Vascular Antibodies	>127 publications with TIMP1 and Vascular
Liver Antibodies	>114 publications with TIMP1 and Liver
Muscle Antibodies	>105 publications with TIMP1 and Muscle
Lung Antibodies	>94 publications with TIMP1 and Lung
Bone Antibodies	>81 publications with TIMP1 and Bone
Heart Antibodies	>78 publications with TIMP1 and Heart
Connective Tissue Antibodies	>63 publications with TIMP1 and Connective Tissue
Embryonic Tissue Antibodies	>57 publications with TIMP1 and Embryonic Tissue
Kidney Antibodies	>57 publications with TIMP1 and Kidney