CNTF ELISA Kit for Rat

Principle of the Assay: The MyBioSource's Mouse RSPO3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse RSPO3 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse RSPO3 coated on a 96-well plate. Standards and samples are pipetted into the wells and RSPO3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse RSPO3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RSPO3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: R-spondin-3 is a protein that in humans is encoded by the RSPO3 gene. This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.

Small volumes of CNTF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS824896 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CNTF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CNTF. The ELISA analytical biochemical technique of the MBS824896 kit is based on CNTF antibody-CNTF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CNTF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CNTF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).