TGF Beta 3 (TGFB3) ELISA Kit for Mouse

Background: Transforming growth factor beta 3 (TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin (CTNNB1) via nuclear phospho-Smad2 and Smad4.

Principle of the Assay: The Mouse TGF beta 3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TGF beta 3 in Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Mouse TGF beta 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TGF beta 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Small volumes of TGFB3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS824950 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TGF beta 3 (TGFB3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TGFB3. The ELISA analytical biochemical technique of the MBS824950 kit is based on TGFB3 antibody-TGFB3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGFB3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGFB3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).