FSTL3 ELISA Kit for Human

Principle of the Assay: MBSHuman Angiopoietin-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin-2 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Angiopoietin-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Angiopoietin-2, ANG-2, is a 496 amino acid secreted protein. After signal sequence processing and maturation, Angiopoietin-2 becomes 478 amino acids long (from 19 to 496). It contains the 1 fibrinogen C-terminal domain which mediates its interaction with TEK/TIE2 receptor. ANG-2 is found to induce tyrosine phosphorylation of TIE2 as well as bind to TIE2 receptor to counteract blood vessel maturation/stability that is mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

Small volumes of FSTL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS825004 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the FSTL3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FSTL3. The ELISA analytical biochemical technique of the MBS825004 kit is based on FSTL3 antibody-FSTL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FSTL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FSTL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).