DKK1 ELISA Kit for Human

Name: Human DKK1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS825042
Price: 640 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS825042

Principle of the assay: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine… More details

$640 / 96 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

DKK1, ELISA Kit

Full Product Name

Human DKK1 ELISA Kit

Product Gene Name

DKK1 elisa kit

Product Synonym Names

Dickkopf-related protein 1; Dickkopf-1; Dkk-1; hDkk-1; SK

Species Reactivity

Human

Samples

Cell Culture Supernatants, Serum, Plasma

Sensitivity

The minimum detectable dose of Human DKK1 is typically less than 10 pg/ml.

Specificity

The Human DKK1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human DKK1 proteins within the range of 62.5 pg/ml - 4000 pg/ml.

Preparation and Storage

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Molecular Weight

28,672 Da

OMIM

605189

3D Structure

ModBase 3D Structure for O94907

Applications Tested/Suitable for DKK1 elisa kit

Sandwich ELISA (SE)

Other Notes

Small volumes of DKK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DKK1 purchase

MBS825042 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DKK1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DKK1. The ELISA analytical biochemical technique of the MBS825042 kit is based on DKK1 antibody-DKK1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DKK1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DKK1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DKK1. It may not necessarily be applicable to this product.

NCBI GI #

7110719

NCBI GeneID

22943

NCBI Accession #

NP_036374.1

UniProt Primary Accession #

O94907

UniProt Secondary Accession #

B2RC19

UniProt Related Accession #

O94907

GenBank Nucleotide #

NM_012242.2

NCBI Official Full Name

dickkopf-related protein 1

NCBI Official Synonym Full Names

dickkopf WNT signaling pathway inhibitor 1

NCBI Official Symbol

DKK1

NCBI Official Synonym Symbols

SK; DKK-1

UniProt Protein Name

Dickkopf-related protein 1

UniProt Synonym Protein Names

Protein Family

Dickkopf-related protein

UniProt Gene Name

DKK1

UniProt Synonym Gene Names

Dickkopf-1; Dkk-1; hDkk-1

UniProt Entry Name

DKK1_HUMAN

NCBI Protein Information

dickkopf-related protein 1; hDkk-1; dickkopf-1 like; dickkopf 1 homolog; dickkopf-like protein 1; dickkopf related protein-1

NCBI Summary for DKK1

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008]

UniProt Comments for DKK1

DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; early endosome membrane; extracellular region; plasma membrane

Molecular Function: signal transducer activity; receptor antagonist activity; protein binding; growth factor activity; low-density lipoprotein receptor binding

Biological Process: negative regulation of skeletal muscle development; cellular morphogenesis during differentiation; negative regulation of Wnt receptor signaling pathway; response to retinoic acid; regulation of receptor internalization; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; mesoderm formation; hair follicle development; negative regulation of mesodermal cell fate specification; endoderm formation; forebrain development; regulation of endodermal cell fate specification; negative regulation of protein complex assembly; embryonic limb morphogenesis

Research Articles on DKK1

1. Serum functional DKK-1 levels showed an independent and negative correlation with radiographic severity of the disease in patients with ankylosing spondylitis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DKK1 elisa kit

Reference Product	PubMed Publications
CTNNB1 elisa kit	>484 publications with DKK1 and CTNNB1
WNT3A elisa kit	>126 publications with DKK1 and WNT3A
LRP5 elisa kit	>119 publications with DKK1 and LRP5
LRP6 elisa kit	>105 publications with DKK1 and LRP6
WNT1 elisa kit	>38 publications with DKK1 and WNT1
KREMEN1 elisa kit	>18 publications with DKK1 and KREMEN1
KREMEN2 elisa kit	>15 publications with DKK1 and KREMEN2
RSPO1 elisa kit	>10 publications with DKK1 and RSPO1
FZD1 elisa kit	>10 publications with DKK1 and FZD1
FZD8 elisa kit	>4 publications with DKK1 and FZD8

Pathways associated with DKK1 elisa kit

Products by Pathway	Pathway Diagram
Cardiac Progenitor Differentiation Pathway antibodies	Cardiac Progenitor Differentiation Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Presenilin Action In Notch And Wnt Signaling Pathway antibodies	Presenilin Action In Notch And Wnt Signaling Pathway Diagram
RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway antibodies	RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway Diagram
Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies	Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By WNT In Cancer Pathway antibodies	Signaling By WNT In Cancer Pathway Diagram
Signaling By Wnt Pathway antibodies	Signaling By Wnt Pathway Diagram
TCF Dependent Signaling In Response To WNT Pathway antibodies	TCF Dependent Signaling In Response To WNT Pathway Diagram

Diseases associated with DKK1 elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>87 publications with DKK1 and Cardiovascular Diseases
Nervous System Diseases Antibodies	>40 publications with DKK1 and Nervous System Diseases
Inflammation Antibodies	>38 publications with DKK1 and Inflammation
Adenocarcinoma Antibodies	>38 publications with DKK1 and Adenocarcinoma
Brain Diseases Antibodies	>36 publications with DKK1 and Brain Diseases
Breast Neoplasms Antibodies	>35 publications with DKK1 and Breast Neoplasms
Necrosis Antibodies	>34 publications with DKK1 and Necrosis
Carcinoma, Hepatocellular Antibodies	>33 publications with DKK1 and Carcinoma, Hepatocellular
Fractures, Bone Antibodies	>32 publications with DKK1 and Fractures, Bone
Neoplasm Metastasis Antibodies	>31 publications with DKK1 and Neoplasm Metastasis

Organs/Tissues associated with DKK1 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>473 publications with DKK1 and Bone
Embryonic Tissue Antibodies	>102 publications with DKK1 and Embryonic Tissue
Bone Marrow Antibodies	>99 publications with DKK1 and Bone Marrow
Brain Antibodies	>79 publications with DKK1 and Brain
Connective Tissue Antibodies	>75 publications with DKK1 and Connective Tissue
Vascular Antibodies	>56 publications with DKK1 and Vascular
Liver Antibodies	>50 publications with DKK1 and Liver
Skin Antibodies	>47 publications with DKK1 and Skin
Muscle Antibodies	>46 publications with DKK1 and Muscle
Lung Antibodies	>41 publications with DKK1 and Lung