CD295 (LEPR) ELISA Kit for Mouse

Name: Mouse LEPR ELISA Kit; CD295; MyBioSource
Brand: MyBioSource
SKU: MBS825084
Price: 615 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS825084

Sandwich ELISA for quantitative detection of Mouse CD295 concentrations. More details

$615 / 96 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

CD295 (LEPR), ELISA Kit

Full Product Name

Mouse CD295 ELISA Kit

Product Gene Name

LEPR elisa kit

Product Synonym Names

DB; OBR; Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD295

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Cell Culture Supernatants, Serum, Plasma

Detection Range

156 pg/ml - 10000 pg/ml

Sensitivity

5 pg/ml

Preparation and Storage

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Molecular Weight

90,887 Da

3D Structure

ModBase 3D Structure for P48356

Detection Method

Colorimetric

Applications Tested/Suitable for LEPR elisa kit

Sandwich ELISA (SE)

Other Notes

Small volumes of LEPR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LEPR purchase

MBS825084 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD295 (LEPR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LEPR. The ELISA analytical biochemical technique of the MBS825084 kit is based on LEPR antibody-LEPR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LEPR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LEPR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for LEPR. It may not necessarily be applicable to this product.

NCBI GI #

171543892

NCBI GeneID

16847

NCBI Accession #

NP_001116371.1

UniProt Primary Accession #

P48356

UniProt Secondary Accession #

O35686; O54986; Q61215; Q64309; Q9QWG3; Q9QWV5

UniProt Related Accession #

P48356

GenBank Nucleotide #

NM_001122899.1

NCBI Official Full Name

leptin receptor isoform 3

NCBI Official Synonym Full Names

leptin receptor

NCBI Official Symbol

Lepr

NCBI Official Synonym Symbols

db; Obr; obl; Leprb; Modb1; LEPROT; OB-RGRP; diabetes; obese-like

UniProt Protein Name

Leptin receptor

UniProt Synonym Protein Names

B219; OB receptor; OB-R; CD_antigen: CD295

Protein Family

Leptin receptor

UniProt Gene Name

Lepr

UniProt Synonym Gene Names

Db; Obr; LEP-R; OB-R

UniProt Entry Name

LEPR_MOUSE

NCBI Protein Information

leptin receptor; B219; LEP-R; OB receptor; leptin receptor gene-related protein

UniProt Comments for LEPR

LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: extracellular space; membrane; integral to plasma membrane; integral to membrane; extracellular region; plasma membrane; receptor complex

Molecular Function: identical protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; transmembrane receptor activity; peptide hormone binding; protein-hormone receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; cholesterol metabolic process; positive regulation of MAPKKK cascade; eating behavior; negative regulation of hydrolase activity; negative regulation of gluconeogenesis; regulation of metabolic process; angiogenesis; signal transduction; leptin-mediated signaling pathway

Research Articles on LEPR

1. Obese diabetic mice lacking leptin receptor (db/db) are deficient in postnatal regenerative osteogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with LEPR elisa kit

Products by Pathway	Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Leptin Insulin Overlap Pathway antibodies	Leptin Insulin Overlap Pathway Diagram
Leptin And Adiponectin Pathway antibodies	Leptin And Adiponectin Pathway Diagram

Diseases associated with LEPR elisa kit

Disease Name	Pubmed Publications
Peripheral Nervous System Diseases Antibodies	>402 publications with LEPR and Peripheral Nervous System Diseases
Obesity Antibodies	>386 publications with LEPR and Obesity
Drug-Related Side Effects and Adverse Reactions Antibodies	>349 publications with LEPR and Drug-Related Side Effects and Adverse Reactions
Overweight Antibodies	>245 publications with LEPR and Overweight
Diabetes Mellitus Antibodies	>214 publications with LEPR and Diabetes Mellitus
Inflammation Antibodies	>206 publications with LEPR and Inflammation
Diabetes Mellitus, Type 2 Antibodies	>123 publications with LEPR and Diabetes Mellitus, Type 2
Insulin Resistance Antibodies	>115 publications with LEPR and Insulin Resistance
Necrosis Antibodies	>110 publications with LEPR and Necrosis
Immunologic Deficiency Syndromes Antibodies	>94 publications with LEPR and Immunologic Deficiency Syndromes

Organs/Tissues associated with LEPR elisa kit

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>1395 publications with LEPR and Skin
Liver Antibodies	>241 publications with LEPR and Liver
Eye Antibodies	>183 publications with LEPR and Eye
Brain Antibodies	>130 publications with LEPR and Brain
Kidney Antibodies	>106 publications with LEPR and Kidney
Heart Antibodies	>91 publications with LEPR and Heart
Spleen Antibodies	>83 publications with LEPR and Spleen
Lung Antibodies	>43 publications with LEPR and Lung
Embryonic Tissue Antibodies	>26 publications with LEPR and Embryonic Tissue
Ovary Antibodies	>20 publications with LEPR and Ovary