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SP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827177

MBS827177 is an antibody targeting SP1. Also known as Anti-SP1 (pT453) Phospho Antibody. Reactive in Human, Mouse, Rat, Bovine, Chicken, Monkey. Host: Rabbit. Rabbit polyclonal antibody to SP1 (pT453) More details

$235 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SP1, Polyclonal Antibody
Full Product Name
Anti-SP1 (pT453) Phospho Antibody
Product Gene Name
anti-SP1 antibody
Product Synonym Names
TSFP1; Transcription factor Sp1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Chicken, Monkey
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of SP1 (pT453) protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
SP1 peptide (MBS8238302) is used for blocking the activity of SP1 antibody (MBS827177)
Molecular Weight
75,824 Da
OMIM
189906
3D Structure
ModBase 3D Structure for P08047
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SP1 (pT453). The exact sequence is proprietary.
Applications Tested/Suitable for anti-SP1 antibody
Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)
Application Notes for anti-SP1 antibody
WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF (1/100 - 1/500), ICC (1/100 - 1/500), ChIP (Use at an assay dependent concentration)

Western Blot (WB) of anti-SP1 antibody

Western blot analysis of ACLY expression in Jurkat (A), Hela (B) whole cell lysates.
Rabbit Anti-SP1 Polyclonal Antibody – Cat# MBS827177

Immunohistochemistry (IHC) of anti-SP1 antibody

Immunohistochemical analysis of ACLY staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-SP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS827177

Immunofluorescence (IF) of anti-SP1 antibody

Immunofluorescent analysis of ACLY staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-SP1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS827177

Other Notes

Small volumes of anti-SP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SP1. It may not necessarily be applicable to this product.

NCBI GI #
352962149
NCBI GeneID
6667
NCBI Accession #
NP_001238754.1
UniProt Primary Accession #
P08047
UniProt Secondary Accession #
Q86TN8; Q9H3Q5; Q9NR51; Q9NY21; Q9NYE7; E4Z9M7; G5E9M8
UniProt Related Accession #
P08047
GenBank Nucleotide #
NM_001251825.1
NCBI Official Full Name
transcription factor Sp1 isoform c
NCBI Official Synonym Full Names
Sp1 transcription factor
NCBI Official Symbol
SP1
UniProt Protein Name
Transcription factor Sp1
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
SP1
UniProt Synonym Gene Names
TSFP1
UniProt Entry Name
SP1_HUMAN
NCBI Protein Information
transcription factor Sp1

NCBI Summary for SP1

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
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UniProt Comments for SP1

SP1: a transcription factor of the Sp1 C2H2-type zinc-finger protein family. Phosphorylated and activated by MAPK. Dephosphorylation by PTEN inhibits DNA binding. Binds to p38 in the nucleus. Interacts with Huntingtin and TAFII130. Transcriptional activity of SP1 and TAFII130 disrupted in early Huntingtin's Disease.

Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein C-terminus binding; histone acetyltransferase binding; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; metal ion binding; histone deacetylase binding; double-stranded DNA binding; bHLH transcription factor binding; transcription factor activity; transcription factor binding

Biological Process: transcription initiation from RNA polymerase II promoter; ossification; embryonic placenta development; transcription, DNA-dependent; viral reproduction; megakaryocyte differentiation; embryonic skeletal development; positive regulation of transcription, DNA-dependent; embryonic process involved in female pregnancy; rhythmic process; cellular lipid metabolic process; enucleate erythrocyte differentiation; liver development; trophectodermal cell differentiation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; embryonic camera-type eye morphogenesis; lung development
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Research Articles on SP1

1. miR-29b functions as a tumor suppressor in TSCC, and the miR-29b/Sp1/PTEN/AKT axis might represent a potential therapeutic target for TSCC intervention

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SP1 antibody

 Reference Product  PubMed Publications
 SP3 antibody  >1261 publications with SP1 and SP3
 HDAC1 antibody  >109 publications with SP1 and HDAC1
 SMAD3 antibody  >86 publications with SP1 and SMAD3
 RELA antibody  >74 publications with SP1 and RELA
 TP53 antibody  >56 publications with SP1 and TP53
 SMAD4 antibody  >41 publications with SP1 and SMAD4
 HDAC2 antibody  >40 publications with SP1 and HDAC2
 EP300 antibody  >21 publications with SP1 and EP300
 ESR1 antibody  >20 publications with SP1 and ESR1
 RB1 antibody  >9 publications with SP1 and RB1

Pathways associated with anti-SP1 antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activation Of Gene Expression By SREBF (SREBP) Pathway antibodies  Activation Of Gene Expression By SREBF (SREBP) Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Corticotropin-releasing Hormone Pathway antibodies  Corticotropin-releasing Hormone Pathway Diagram

Diseases associated with anti-SP1 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >365 publications with SP1 and Breast Neoplasms
 Nervous System Diseases Antibodies  >301 publications with SP1 and Nervous System Diseases
 Liver Diseases Antibodies  >276 publications with SP1 and Liver Diseases
 Necrosis Antibodies  >273 publications with SP1 and Necrosis
 Cardiovascular Diseases Antibodies  >237 publications with SP1 and Cardiovascular Diseases
 Inflammation Antibodies  >235 publications with SP1 and Inflammation
 Disease Models, Animal Antibodies  >216 publications with SP1 and Disease Models, Animal
 Liver Neoplasms Antibodies  >210 publications with SP1 and Liver Neoplasms
 Neoplasms, Experimental Antibodies  >194 publications with SP1 and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >190 publications with SP1 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-SP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >330 publications with SP1 and Bone
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