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TNNI3K Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829769

MBS829769 is an antibody targeting TNNI3K. Also known as Anti-CARK Antibody. Reactive in Human, Mouse, Rat, Bovine. Host: Rabbit. Rabbit polyclonal antibody to CARK More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CARK (TNNI3K), Polyclonal Antibody
Full Product Name
Anti-CARK Antibody
Product Gene Name
Product Synonym Names
CARK; Serine/threonine-protein kinase TNNI3K; Cardiac ankyrin repeat kinase; Cardiac troponin I-interacting kinase; TNNI3-interacting kinase
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of CARK protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
CARK peptide (MBS8207280) is used for blocking the activity of CARK antibody (MBS829769)
Molecular Weight
94,517 Da
OMIM
613932
3D Structure
ModBase 3D Structure for Q59H18
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CARK. The exact sequence is proprietary.
Applications Tested/Suitable for anti-TNNI3K antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-TNNI3K antibody
WB (1/500 - 1/1000), IHC (1/100 - 1/200)

Western Blot (WB) of anti-TNNI3K antibody

Western blot analysis of CARK expression in HEK293T (A), Raw264.7 (B), H9C2 (C) whole cell lysates.
Rabbit Anti-TNNI3K Polyclonal Antibody – Cat# MBS829769

Immunohistochemistry (IHC) of anti-TNNI3K antibody

Immunohistochemical analysis of CARK staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-TNNI3K Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS829769

Other Notes

Small volumes of anti-TNNI3K antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TNNI3K. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
FPGT-TNNI3K fusion protein isoform b
NCBI Official Synonym Full Names
FPGT-TNNI3K readthrough
NCBI Official Symbol
FPGT-TNNI3K
UniProt Protein Name
Serine/threonine-protein kinase TNNI3K
UniProt Synonym Protein Names
Cardiac ankyrin repeat kinase; Cardiac troponin I-interacting kinase; TNNI3-interacting kinase
UniProt Gene Name
TNNI3K
UniProt Entry Name
TNI3K_HUMAN
NCBI Protein Information
FPGT-TNNI3K fusion protein

NCBI Summary for TNNI3K

This locus represents naturally occurring read-through transcription from the neighboring fucose-1-phosphate guanylyltransferase (FPGT) and TNNI3 interacting kinase (TNNI3K) genes. Alternative splicing results in multiple transcript variants that are composed of in-frame exons from each individual gene. [provided by RefSeq, Dec 2010]
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UniProt Comments for TNNI3K

TNNI3K: May play a role in cardiac physiology. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; MLK family; HH498 subfamily

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein C-terminus binding; protein serine/threonine kinase activity; protein binding; troponin I binding; metal ion binding; ATP binding; protein kinase activity

Biological Process: regulation of heart rate; protein amino acid phosphorylation

Disease: Cardiac Conduction Disease With Or Without Dilated Cardiomyopathy
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TNNI3K antibody

 Reference Product  PubMed Publications
 LRRIQ3 antibody  >1 publications with TNNI3K and LRRIQ3
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