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Histone H4 (HIST1H4A) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829794

MBS829794 is an antibody targeting HIST1H4A. Also known as Anti-Histone H4 (AcK8) Acetyl Antibody. Reactive in Human, Mouse, Rat, Bovine, Chicken, Monkey, Porcine. Host: Rabbit. Rabbit polyclonal antibody to Histone H4 (AcK8) More details

$235 / 0.03 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Histone H4 (HIST1H4A), Polyclonal Antibody
Full Product Name
Anti-Histone H4 (AcK8) Acetyl Antibody
Product Gene Name
anti-HIST1H4A antibody
Product Synonym Gene Name
HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST4H4
Product Synonym Names
H4/A; H4FA; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH; H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; HIST2H4; H4/O; H4FO; Histone H4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Chicken, Monkey, Porcine
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of Histone H4 (AcK8) protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
Histone H4 peptide (MBS8206792) is used for blocking the activity of Histone H4 antibody (MBS829794)
Molecular Weight
11,367 Da
OMIM
142750
3D Structure
ModBase 3D Structure for P62805
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H4 (AcK8). The exact sequence is proprietary.
Applications Tested/Suitable for anti-HIST1H4A antibody
Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-HIST1H4A antibody
WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF (1/100 - 1/500), ICC (1/100 - 1/500)

Western Blot (WB) of anti-HIST1H4A antibody

Western blot analysis of Histone H4 (AcK8) expression in HeLa TSA-treated (A) whole cell lysates.
Rabbit Anti-HIST1H4A Polyclonal Antibody – Cat# MBS829794

Immunohistochemistry (IHC) of anti-HIST1H4A antibody

Immunohistochemical analysis of Histone H4 (AcK8) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-HIST1H4A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS829794

Immunofluorescence (IF) of anti-HIST1H4A antibody

Immunofluorescent analysis of Histone H4 (AcK8) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-HIST1H4A Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS829794

Other Notes

Small volumes of anti-HIST1H4A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HIST1H4A. It may not necessarily be applicable to this product.

NCBI GI #
77539758
NCBI GeneID
554313
NCBI Accession #
NP_001029249.1
UniProt Primary Accession #
P62805
UniProt Secondary Accession #
P02304; P02305; Q6DRA9; Q6FGB8; Q6NWP7; A2VCL0
UniProt Related Accession #
P62805
GenBank Nucleotide #
NM_001034077.4
NCBI Official Full Name
histone H4
NCBI Official Synonym Full Names
histone cluster 2, H4b
NCBI Official Symbol
HIST2H4B
NCBI Official Synonym Symbols
H4/o
UniProt Protein Name
Histone H4
Protein Family
Histone H4-like protein
UniProt Gene Name
HIST1H4A
UniProt Synonym Gene Names
H4/A; H4FA
UniProt Entry Name
H4_HUMAN
NCBI Protein Information
histone H4

NCBI Summary for HIST1H4A

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Aug 2015]
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UniProt Comments for HIST1H4A

H4: histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The gene encoding H4 is intronless.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; DNA replication-independent nucleosome assembly at centromere; DNA replication-independent nucleosome assembly; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; mitotic cell cycle; DNA methylation on cytosine; DNA replication-dependent nucleosome assembly; telomere maintenance; regulation of gene expression, epigenetic; negative regulation of megakaryocyte differentiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-HIST1H4A antibody

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Organs/Tissues associated with anti-HIST1H4A antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with HIST1H4A and Testis
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