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GTF2F1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS831391

MBS831391 is an antibody targeting GTF2F1. Also known as GTF2F1 antibody. Host: Mouse. Mouse monoclonal GTF2F1 antibody More details

$1625 / 0.1 mL +2 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GTF2F1, Monoclonal Antibody
Full Product Name
GTF2F1 antibody
Product Gene Name
anti-GTF2F1 antibody
Product Synonym Names
Monoclonal GTF2F1; Anti-GTF2F1; General transcription factor IIF polypeptide 1; RAP74; Transcription initiation factor RAP74; TFIIF alpha; GTF2F1; GTFF1 2; GTFF1-2
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4B10
Host
Mouse
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purity/Purification
GTF2F1 antibody was purified by affinity chromatography.
Concentration
500 ug-1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
58,240 Da
OMIM
189968
3D Structure
ModBase 3D Structure for P35269
Cross-Reactivity
Human, Dog, Monkey
Immunogen
GTF2F1 antibody was raised in mouse using a full length recombinant protein of human GTF2F1 (NP_002087) produced in HEK293T cells, as the immunogen.

Product Categories/Family for anti-GTF2F1 antibody

DNA & RNA
Applications Tested/Suitable for anti-GTF2F1 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-GTF2F1 antibody
FC: 1:100
IHC: 1:150
WB: 1:500-2000

Western Blot (WB) of anti-GTF2F1 antibody

Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GTF2F1 protein (Right) or empty vector (Left) detected with GTF2F1 antibody
Mouse Anti-GTF2F1 Monoclonal Antibody – Cat# MBS831391

Immunohistochemistry (IHC) of anti-GTF2F1 antibody

Immunohistochemical analysis of GTF2F1 protein in paraffin embedded Human endometrium tissue using GTF2F1 antibody
Mouse Anti-GTF2F1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS831391

Other Notes

Small volumes of anti-GTF2F1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GTF2F1. It may not necessarily be applicable to this product.

NCBI GI #
156104891
NCBI GeneID
2962
NCBI Accession #
NP_002087
UniProt Primary Accession #
P35269
UniProt Secondary Accession #
Q9BWN0; B2RCS0
UniProt Related Accession #
P35269
GenBank Nucleotide #
NM_002096.2
NCBI Official Full Name
general transcription factor IIF subunit 1
NCBI Official Synonym Full Names
general transcription factor IIF, polypeptide 1, 74kDa
NCBI Official Symbol
GTF2F1
NCBI Official Synonym Symbols
BTF4; RAP74; TF2F1; TFIIF
UniProt Protein Name
General transcription factor IIF subunit 1
UniProt Synonym Protein Names
General transcription factor IIF 74 kDa subunit; Transcription initiation factor IIF subunit alpha; TFIIF-alpha; Transcription initiation factor RAP74
Protein Family
General transcription factor
UniProt Gene Name
GTF2F1
UniProt Synonym Gene Names
RAP74; TFIIF-alpha
UniProt Entry Name
T2FA_HUMAN
NCBI Protein Information
general transcription factor IIF subunit 1

UniProt Comments for GTF2F1

GTF2F1: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the TFIIF alpha subunit family.

Protein type: Kinase, protein; Transcription initiation complex; EC 2.7.11.1; ATYPICAL group; GTF2F1 family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; transcription factor TFIIF complex; cell junction; nucleus

Molecular Function: protein binding; DNA binding; phosphatase activator activity; transcription coactivator activity; catalytic activity; transcription factor binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; positive regulation of catalytic activity; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; positive regulation of viral transcription; positive regulation of RNA elongation from RNA polymerase II promoter; RNA splicing; response to virus; RNA elongation from RNA polymerase II promoter; gene expression
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Research Articles on GTF2F1

1. These results suggest that Mediator structural shifts induced by activator binding help stably orient pol II prior to transcription initiation within the human mediator-RNA polymerase II-TFIIF assembly.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GTF2F1 antibody

 Reference Product  PubMed Publications
 GTF2F2 antibody  >2 publications with GTF2F1 and GTF2F2
 TBP antibody  >1 publications with GTF2F1 and TBP
 GTF2B antibody  >1 publications with GTF2F1 and GTF2B

Pathways associated with anti-GTF2F1 antibody

 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies  Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram
 Formation Of The Early Elongation Complex Pathway antibodies  Formation Of The Early Elongation Complex Pathway Diagram
 Formation Of The HIV-1 Early Elongation Complex Pathway antibodies  Formation Of The HIV-1 Early Elongation Complex Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
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