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PPME1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS832543

MBS832543 is an antibody targeting PPME1. Also known as PPME1 antibody. Host: Mouse. Mouse monoclonal PPME1 antibody More details

$1615 / 0.1 mL +2 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PPME1, Monoclonal Antibody
Full Product Name
PPME1 antibody
Product Gene Name
Product Synonym Names
Monoclonal PPME1; Anti-PPME1; PPME1; PPME 1; PPME-1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10B2
Host
Mouse
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purity/Purification
PPME1 antibody was purified by affinity chromatography.
Concentration
500 ug-1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
43,870 Da
OMIM
611117
3D Structure
ModBase 3D Structure for Q9Y570
Cross-Reactivity
Human
Immunogen
PPME1 antibody was raised in mouse using a full length recombinant protein of human PPME1 (NP_057231) produced in HEK293T cells, as the immunogen.

Product Categories/Family for anti-PPME1 antibody

Applications Tested/Suitable for anti-PPME1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-PPME1 antibody
FC: 1:100
IF: 1:100
WB: 1:2000

Flow Cytometry of anti-PPME1 antibody

Flow Cytometric analysis using PPME1 antibody
Flow Cytometric analysis of HEK293T cells transfected with either recombinant PPME1 protein (red) or empty vector (blue) stained using PPME1 antibody
Mouse Anti-PPME1 Monoclonal Antibody – Cat# MBS832543

Immunofluorescence of anti-PPME1 antibody

Immunofluorescent staining using PPME1 antibody
Immunofluorescent staining of COS7 cells transiently transfected with recombinant PPME1 protein using PPME1 antibody
Mouse Anti-PPME1 Monoclonal Antibody, Immunofluorescence – Cat# MBS832543

Western Blot of anti-PPME1 antibody

Western Blot analysis using PPME1 antibody
Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PPME1 protein (Right) or empty vector (Left) detected with PPME1 antibody
Mouse Anti-PPME1 Monoclonal Antibody, Western Blot – Cat# MBS832543

Other Notes

Small volumes of anti-PPME1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPME1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein phosphatase methylesterase 1 isoform a
NCBI Official Synonym Full Names
protein phosphatase methylesterase 1
NCBI Official Symbol
PPME1
NCBI Official Synonym Symbols
PME-1
UniProt Protein Name
Protein phosphatase methylesterase 1
Protein Family
UniProt Gene Name
PPME1
UniProt Synonym Gene Names
PME1; PME-1
UniProt Entry Name
PPME1_HUMAN
NCBI Protein Information
protein phosphatase methylesterase 1

NCBI Summary for PPME1

This gene encodes a protein phosphatase methylesterase localized to the nucleus. The encoded protein acts on the protein phosphatase-2A catalytic subunit and supports the ERK pathway through dephosphorylation of regulatory proteins. It plays a role in malignant glioma progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]
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UniProt Comments for PPME1

PPME1: Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme. Belongs to the AB hydrolase superfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.89; Hydrolase; Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 11q13.4

Molecular Function: protein phosphatase 2A binding; protein phosphatase type 2A regulator activity; protein C-terminal methylesterase activity; protein phosphatase inhibitor activity; protein phosphatase binding

Biological Process: regulation of catalytic activity; negative regulation of catalytic activity; protein amino acid demethylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPME1 antibody

Diseases associated with anti-PPME1 antibody

Organs/Tissues associated with anti-PPME1 antibody

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