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NNMT Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS832869

MBS832869 is an antibody targeting NNMT. Also known as NNMT antibody. Host: Mouse. Mouse monoclonal NNMT antibody More details

$1730 / 0.1 mL +2 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NNMT, Monoclonal Antibody
Full Product Name
NNMT antibody
Product Gene Name
Product Synonym Names
Monoclonal NNMT; Anti-NNMT; Nicotinamide N-Methyltransferase
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3D8
Host
Mouse
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purity/Purification
NNMT antibody was purified by affinity chromatography.
Concentration
500 ug-1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
29,574 Da
OMIM
600008
3D Structure
ModBase 3D Structure for P40261
Cross-Reactivity
Human, Mouse
Immunogen
NNMT antibody was raised in mouse using a full length recombinant protein of human NNMT (NP_006160) produced in HEK293T cells, as the immunogen.

Product Categories/Family for anti-NNMT antibody

Applications Tested/Suitable for anti-NNMT antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-NNMT antibody
IF: 1:100
FC: 1:100
IHC: 1:150
WB: 1:500-2000

Flow Cytometry (FC/FACS) of anti-NNMT antibody

Flow Cytometric analysis of Jurkat cells using NNMT antibody (Red), compared to a nonspecific negative control antibody (Blue).
Mouse Anti-NNMT Monoclonal Antibody – Cat# MBS832869

Western Blot (WB) of anti-NNMT antibody

Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NNMT protein (Right) or empty vector (Left) detected with NNMT antibody
Mouse Anti-NNMT Monoclonal Antibody, Western Blot (WB) – Cat# MBS832869

Immunohistochemistry (IHC) of anti-NNMT antibody

Immunohistochemical analysis of NNMT protein in paraffin embedded Human pancreas tissue using NNMT antibody
Mouse Anti-NNMT Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS832869

Immunofluorescence (IF) of anti-NNMT antibody

Immunofluorescent staining of COS7 cells transiently transfected with recombinant NNMT protein using NNMT antibody
Mouse Anti-NNMT Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS832869

Other Notes

Small volumes of anti-NNMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NNMT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nicotinamide N-methyltransferase
NCBI Official Synonym Full Names
nicotinamide N-methyltransferase
NCBI Official Symbol
NNMT
UniProt Protein Name
Nicotinamide N-methyltransferase
UniProt Gene Name
NNMT
UniProt Entry Name
NNMT_HUMAN
NCBI Protein Information
nicotinamide N-methyltransferase

NCBI Summary for NNMT

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]

UniProt Comments for NNMT

NNMT: Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Belongs to the NNMT/PNMT/TEMT family.

Protein type: Methyltransferase; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.1.1.1

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytosol

Molecular Function: nicotinamide N-methyltransferase activity

Biological Process: methylation; response to drug; organ regeneration; xenobiotic metabolic process; response to organic nitrogen
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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