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Alpha-1-Antitrypsin (a1AT) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2001932
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MBS2001932 is an antibody targeting a1AT. Also known as Polyclonal Antibody to Alpha-1-Antitrypsin (a1AT). Reactive in Human, Pig. Host: Rabbit. More details

$210 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Alpha-1-Antitrypsin (a1AT), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Alpha-1-Antitrypsin (a1AT)
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Pig
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against a1AT. It has been selected for its ability to recognize a1AT in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: a1AT (MBS2001932)
Biotin Conjugated Antibody: a1AT (MBS2006397)
Matching Pairs
Unconjugated Antibody: a1AT (MBS2001932)
Immunogen: a1AT (MBS2029530)
Molecular Weight
34,755 Da
OMIM
K01396 mRNA
3D Structure
ModBase 3D Structure for P01009
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen Information
Alpha 1-Antitrypsin (A1AT) is a protease inhibitor belonging to the serpin superfamily. It is generally known as serum trypsin inhibitor. It protects tissues from enzymes of inflammatory cells, especially neutrophil elastase, and has a reference range in blood of 1.5~3.5 gram/liter. In its absence, neutrophil elastase is free to break down elastin, which contributes to the elasticity of the lungs, resulting in respiratory complications such as emphysema, or COPD in adults and cirrhosis in adults or children. A1AT is a 52-kDa serpin and, in medicine, it is considered the most prominent serpin; the terms α1-antitrypsin and protease inhibitor (Pi) are often used interchangeably.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2058027
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-a1AT antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-a1AT antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-a1AT antibody

Western Blot: Sample: Human Serum.
Rabbit Anti-a1AT Polyclonal Antibody – Cat# MBS2001932

Western Blot (WB) of anti-a1AT antibody

Western Blot: Sample: Recombinant a1AT, Human.
Rabbit Anti-a1AT Polyclonal Antibody, Western Blot (WB) – Cat# MBS2001932

Immunohistochemistry (IHC) of anti-a1AT antibody

DAB staining on IHC-P; Samples: Human Pancreas Tissue.
Rabbit Anti-a1AT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001932

Immunohistochemistry (IHC) of anti-a1AT antibody

DAB staining on IHC-P; Samples: Human Liver Tissue
Rabbit Anti-a1AT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001932

Immunohistochemistry (IHC) of anti-a1AT antibody

DAB staining on IHC-P; Samples: Human Stomach Tissue
Rabbit Anti-a1AT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001932

Other Notes

Small volumes of anti-a1AT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for a1AT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-1-antitrypsin
NCBI Official Synonym Full Names
serpin family A member 1
NCBI Official Symbol
SERPINA1
NCBI Official Synonym Symbols
PI; A1A; AAT; PI1; A1AT; nNIF; PRO2275; alpha1AT
UniProt Protein Name
Alpha-1-antitrypsin
UniProt Synonym Protein Names
Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1
UniProt Gene Name
SERPINA1
UniProt Synonym Gene Names
AAT; PI; SPAAT
NCBI Protein Information
alpha-1-antitrypsin

NCBI Summary for a1AT

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for a1AT

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-a1AT antibody

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