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NEK6 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS833489

MBS833489 is an antibody targeting NEK6. Also known as NEK6 antibody. Host: Mouse. Mouse monoclonal NEK6 antibody More details

$1615 / 0.1 mL +2 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NEK6, Monoclonal Antibody
Full Product Name
NEK6 antibody
Product Gene Name
anti-NEK6 antibody
Product Synonym Names
Monoclonal NEK6; Anti-NEK6; Nima; Never In Mitosis Gene A-Related Kinase 6; SID6-1512; NEK6; NEK 6; NEK-6
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5D7
Host
Mouse
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purity/Purification
NEK6 antibody was purified by affinity chromatography.
Concentration
500 ug-1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
38,460 Da
OMIM
604884
3D Structure
ModBase 3D Structure for Q9HC98
Cross-Reactivity
Human
Immunogen
NEK6 antibody was raised in mouse using a protein expressed in 293T cell transfected with human NEK6 expression vector as the immunogen.

Product Categories/Family for anti-NEK6 antibody

Cell Cycle & Cell Death
Applications Tested/Suitable for anti-NEK6 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-NEK6 antibody
IF: 1:100
IHC: 1:50
WB: 1:2000

Western Blot (WB) of anti-NEK6 antibody

Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NEK6 protein (Right) or empty vector (Left) detected with NEK6 antibody
Mouse Anti-NEK6 Monoclonal Antibody – Cat# MBS833489

Immunohistochemistry (IHC) of anti-NEK6 antibody

Immunohistochemical analysis of NEK6 protein in paraffin embedded Adenocarcinoma of Human colon tissue using NEK6 antibody
Mouse Anti-NEK6 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS833489

Immunofluorescence (IF) of anti-NEK6 antibody

Immunofluorescent staining of COS7 cells transiently transfected with recombinant NEK6 protein using NEK6 antibody
Mouse Anti-NEK6 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS833489

Other Notes

Small volumes of anti-NEK6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEK6. It may not necessarily be applicable to this product.

NCBI GI #
49457438
NCBI GeneID
10783
NCBI Accession #
CAG47018.1
UniProt Primary Accession #
Q9HC98
UniProt Secondary Accession #
Q5TBG3; Q5TBG9; Q6FG86; Q6IAR3; Q96E83; Q9ULX2; B7Z2D9
UniProt Related Accession #
Q9HC98
NCBI Official Full Name
NEK6, partial
NCBI Official Synonym Full Names
NIMA-related kinase 6
NCBI Official Symbol
NEK6
NCBI Official Synonym Symbols
SID6-1512
UniProt Protein Name
Serine/threonine-protein kinase Nek6
UniProt Synonym Protein Names
Never in mitosis A-related kinase 6; NimA-related protein kinase 6; Protein kinase SID6-1512
Protein Family
Serine/threonine-protein kinase
UniProt Gene Name
NEK6
UniProt Synonym Gene Names
NimA-related protein kinase 6
UniProt Entry Name
NEK6_HUMAN
NCBI Protein Information
serine/threonine-protein kinase Nek6

NCBI Summary for NEK6

The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for NEK6

NEK6: a protein kinase of the NEK family. Important in mitosis. May regulate spindle function.

Protein type: Protein kinase, Other; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; NEK family

Chromosomal Location of Human Ortholog: 9q33.3-q34.11

Cellular Component: spindle pole; microtubule; cytoplasm; microtubule organizing center; nuclear speck; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; signal transducer activity; protein binding; kinesin binding; magnesium ion binding; protein kinase binding; ATP binding

Biological Process: mitosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; protein amino acid autophosphorylation; regulation of mitotic metaphase/anaphase transition; regulation of mitotic cell cycle; mitotic nuclear envelope disassembly; cytokinesis; spindle assembly; signal transduction; protein amino acid phosphorylation; chromosome segregation; peptidyl-serine phosphorylation; mitotic cell cycle; G2/M transition DNA damage checkpoint
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Research Articles on NEK6

1. Nek6 plays an important role in the premature senescence of cancer cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NEK6 antibody

 Reference Product  PubMed Publications
 NEK9 antibody  >14 publications with NEK6 and NEK9
 KIF11 antibody  >2 publications with NEK6 and KIF11
 PLK1 antibody  >2 publications with NEK6 and PLK1

Pathways associated with anti-NEK6 antibody

 Products by Pathway  Pathway Diagram
 Activation Of NIMA Kinases NEK9, NEK6, NEK7 Pathway antibodies  Activation Of NIMA Kinases NEK9, NEK6, NEK7 Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Prophase Pathway antibodies  Mitotic Prophase Pathway Diagram
 Nuclear Envelope Breakdown Pathway antibodies  Nuclear Envelope Breakdown Pathway Diagram
 Nuclear Pore Complex (NPC) Disassembly Pathway antibodies  Nuclear Pore Complex (NPC) Disassembly Pathway Diagram

Diseases associated with anti-NEK6 antibody

 Disease Name  Pubmed Publications
 Carcinoma, Hepatocellular Antibodies  >5 publications with NEK6 and Carcinoma, Hepatocellular
 Cell Transformation, Neoplastic Antibodies  >4 publications with NEK6 and Cell Transformation, Neoplastic
 Liver Diseases Antibodies  >4 publications with NEK6 and Liver Diseases
 Liver Neoplasms Antibodies  >4 publications with NEK6 and Liver Neoplasms
 Neoplasms, Experimental Antibodies  >2 publications with NEK6 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >2 publications with NEK6 and Lung Neoplasms
 Fibrosis Antibodies  >1 publications with NEK6 and Fibrosis
 Hypertrophy Antibodies  >1 publications with NEK6 and Hypertrophy
 Liver Neoplasms, Experimental Antibodies  >1 publications with NEK6 and Liver Neoplasms, Experimental
 Skin Neoplasms Antibodies  >1 publications with NEK6 and Skin Neoplasms

Organs/Tissues associated with anti-NEK6 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >7 publications with NEK6 and Liver
 Lung Antibodies  >3 publications with NEK6 and Lung
 Spleen Antibodies  >2 publications with NEK6 and Spleen
 Muscle Antibodies  >2 publications with NEK6 and Muscle
 Heart Antibodies  >2 publications with NEK6 and Heart
 Embryonic Tissue Antibodies  >2 publications with NEK6 and Embryonic Tissue
 Brain Antibodies  >2 publications with NEK6 and Brain
 Uterus Antibodies  >1 publications with NEK6 and Uterus
 Testis Antibodies  >1 publications with NEK6 and Testis
 Stomach Antibodies  >1 publications with NEK6 and Stomach
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