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SSEA4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460512

Background: SSEA-4 is stage-specific embryonic antigen 4 (SSEA4) is a cell surface antigen, expressed along with SSEA3, TRA1-60 and TRA1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these… More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SSEA4, Polyclonal Antibody
Full Product Name
Rabbit anti SSEA4 Polyclonal Antibody
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Purity/Purification
Epitope Affinity Purification
Specificity
This antibody recognizes human SSEA4 protein. The other species are not tested.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C.
The antibodies can be stored at 2 degree C to 8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
>200
Preparation
A synthetic peptide derived from C-Terminus of human SSEA-4 protein.
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Applications Tested/Suitable for anti-SSEA4 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA), Immunoprecipitation (IP)
Application Notes for anti-SSEA4 antibody
Immunohistochemistry (IHC): 2-10 ug/ml
Western Blot (WB): 0.1-1 ug/ml
ELISA (EIA): 0.01-0.1 ug/ml
Immunoprecipitation (IP): 2-5 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.

Immunohistochemistry (IHC) of anti-SSEA4 antibody

Immunohistochemistry: The whole cell pellet K562 (FFPE) stained with Rabbit anti-SSEA4 antibody (Cat# 620-500) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Rabbit Anti-SSEA4 Polyclonal Antibody – Cat# MBS460512

Other Notes

Small volumes of anti-SSEA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SSEA4 purchase

MBS460512 is an antibody targeting SSEA4. Also known as Rabbit anti SSEA4 Polyclonal Antibody. Reactive in Human. Host: Rabbit.
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