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PDLIM2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS834453

MBS834453 is an antibody targeting PDLIM2. Also known as PDLIM2 antibody. Host: Mouse. Mouse monoclonal PDLIM2 antibody More details

$1615 / 0.1 mL +2 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PDLIM2, Monoclonal Antibody
Full Product Name
PDLIM2 antibody
Product Gene Name
Product Synonym Names
Monoclonal PDLIM2; Anti-PDLIM2; FLJ34715; MYSTIQUE; SLIM; PDLIM2; PDLIM 2; PDLIM-2
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10C4
Host
Mouse
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purity/Purification
PDLIM2 antibody was purified by affinity chromatography.
Concentration
0.5-1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
62,746 Da
OMIM
609722
3D Structure
ModBase 3D Structure for Q96JY6
Cross-Reactivity
Human, Dog, Monkey
Immunogen
PDLIM2 antibody was raised in Mouse using full length human recombinant protein of human PDLIM2 produced in HEK293T cell as the immunogen.

Product Categories/Family for anti-PDLIM2 antibody

Applications Tested/Suitable for anti-PDLIM2 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-PDLIM2 antibody
IF: 1:100
IHC: 1:150
WB: 1:500-2000

Immunofluorescence (IF) of anti-PDLIM2 antibody

Immunofluorescent staining of COS7 cells transiently transfected with recombinant PDLIM2 protein using PDLIM2 antibody
Mouse Anti-PDLIM2 Monoclonal Antibody – Cat# MBS834453

Western Blot (WB) of anti-PDLIM2 antibody

Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PDLIM2 protein (Right) or empty vector (Left) detected with PDLIM2 antibody
Mouse Anti-PDLIM2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS834453

Immunohistochemistry (IHC) of anti-PDLIM2 antibody

Immunohistochemical analysis of PDLIM2 protein in paraffin embedded Human tonsil tissue using PDLIM2 antibody
Mouse Anti-PDLIM2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS834453

Other Notes

Small volumes of anti-PDLIM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PDLIM2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
PDZ and LIM domain protein 2 isoform 2
NCBI Official Synonym Full Names
PDZ and LIM domain 2 (mystique)
NCBI Official Symbol
PDLIM2
NCBI Official Synonym Symbols
SLIM; MYSTIQUE
UniProt Protein Name
PDZ and LIM domain protein 2
UniProt Synonym Protein Names
PDZ-LIM protein mystique
UniProt Gene Name
PDLIM2
UniProt Entry Name
PDLI2_HUMAN
NCBI Protein Information
PDZ and LIM domain protein 2

NCBI Summary for PDLIM2

This gene encodes a member of the ALP subfamily of PDZ-LIM domain proteins. The encoded protein suppresses anchorage-dependent growth and promotes cell migration and adhesion through interactions with the actin cytoskeleton via the PDZ domain. The encoded protein is also a putative tumor suppressor protein, and decreased expression of this gene is associated with several malignancies including breast cancer and adult T-cell leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for PDLIM2

PDLIM2: Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity. Interacts with alpha-actinins ACTN1 and ACTN4, FLNA and MYH9. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Cell adhesion

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: cortical actin cytoskeleton; focal adhesion; stress fiber; nucleus; actin cytoskeleton

Molecular Function: zinc ion binding; filamin binding; muscle alpha-actinin binding; myosin heavy chain binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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