• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NIT1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS834577

MBS834577 is an antibody targeting NIT1. Also known as NIT1 antibody. Host: Mouse. Mouse monoclonal NIT1 antibody More details

$1615 / 0.1 mL +2 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NIT1, Monoclonal Antibody
Full Product Name
NIT1 antibody
Product Gene Name
Product Synonym Names
Monoclonal NIT1; Anti-NIT1; Nitrilase 1; MGC57670; NIT1; NIT 1; NIT-1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4D12
Host
Mouse
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purity/Purification
NIT1 antibody was purified by affinity chromatography.
Concentration
500 ug-1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
26,326 Da
OMIM
604618
3D Structure
ModBase 3D Structure for Q86X76
Cross-Reactivity
Human
Immunogen
NIT1 antibody was raised in mouse using a full length recombinant protein of human NIT1 (NP_005591) produced in HEK293T cells, as the immunogen.

Product Categories/Family for anti-NIT1 antibody

Applications Tested/Suitable for anti-NIT1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-NIT1 antibody
FC: 1:100
IF: 1:100
IHC: 1:50
WB: 1:1000

Flow Cytometry (FC/FACS) of anti-NIT1 antibody

Flow Cytometric analysis of HEK293T cells transfected with either recombinant NIT1 protein (red) or empty vector (blue) stained using NIT1 antibody
Mouse Anti-NIT1 Monoclonal Antibody – Cat# MBS834577

Western Blot (WB) of anti-NIT1 antibody

Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NIT1 protein (Right) or empty vector (Left) detected with NIT1 antibody
Mouse Anti-NIT1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS834577

Immunohistochemistry (IHC) of anti-NIT1 antibody

Immunohistochemical analysis of NIT1 protein in paraffin embedded Adenocarcinoma of Human ovary tissue using NIT1 antibody
Mouse Anti-NIT1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS834577

Immunofluorescence (IF) of anti-NIT1 antibody

Immunofluorescent staining of COS7 cells transiently transfected with recombinant NIT1 protein using NIT1 antibody
Mouse Anti-NIT1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS834577

Other Notes

Small volumes of anti-NIT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NIT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nitrilase homolog 1 isoform 1
NCBI Official Synonym Full Names
nitrilase 1
NCBI Official Symbol
NIT1
UniProt Protein Name
Nitrilase homolog 1
Protein Family
UniProt Gene Name
NIT1
UniProt Entry Name
NIT1_HUMAN
NCBI Protein Information
nitrilase homolog 1

NCBI Summary for NIT1

This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Show more

UniProt Comments for NIT1

NIT1: Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2. Belongs to the UPF0012 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.-.-; Hydrolase

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: mitochondrion; nucleus

Molecular Function: nitrilase activity

Biological Process: nitrogen compound metabolic process
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000