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CD180 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS834605

MBS834605 is an antibody targeting CD180. Also known as CD180 antibody (PE). Host: Rat. Rat monoclonal CD180 antibody (PE) More details

$780 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD180, Monoclonal Antibody
Full Product Name
CD180 antibody (PE)
Product Gene Name
Product Synonym Names
Monoclonal CD180; Anti-CD180; CD-180; CD180; CD 180; LY-78; Toll-like Receptor; RP-105; TLR Family
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
RP/14
Host
Rat
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
200 ug/ml (lot specific)
Specificity
Mouse
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Molecular Weight
74,179 Da
OMIM
602226
3D Structure
ModBase 3D Structure for Q99467
Tag/Conjugate
PE
Applications Tested/Suitable for anti-CD180 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD180 antibody
FC: <= 1 ug/test

A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, longer incubations appear to help stain the population; therefore we recommend a 60 minute incubation at 4 degree C.

Flow Cytometry (FC/FACS) of anti-CD180 antibody

Staining of BALB/c splenocytes with 0.06 ug of Rat IgG2a kappa Isotype Control (PE) (open histogram) or 0.125 ug of CD180 antibody (PE) (filled histogram). Total viable cells were used for analysis.
Rat Anti-CD180 Monoclonal Antibody – Cat# MBS834605

Other Notes

Small volumes of anti-CD180 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD180. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD180 antigen
NCBI Official Synonym Full Names
CD180 molecule
NCBI Official Symbol
CD180
NCBI Official Synonym Symbols
LY64; Ly78; RP105
UniProt Protein Name
CD180 antigen
UniProt Synonym Protein Names
Lymphocyte antigen 64; Radioprotective 105 kDa protein; CD_antigen: CD180
Protein Family
UniProt Gene Name
CD180
UniProt Synonym Gene Names
LY64; RP105
UniProt Entry Name
CD180_HUMAN
NCBI Protein Information
CD180 antigen

NCBI Summary for CD180

CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD180

CD180: May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: integral to membrane; plasma membrane

Biological Process: positive regulation of lipopolysaccharide-mediated signaling pathway; innate immune response; inflammatory response; B cell proliferation during immune response
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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