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Creatine Kinase (CKM) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS835053

MBS835053 is an antibody targeting CKM. Also known as Creatine kinase antibody. Host: Rabbit. Affinity purified Rabbit polyclonal Creatine kinase antibody More details

$1565 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Creatine kinase (CKM), Polyclonal Antibody
Full Product Name
Creatine kinase antibody
Product Gene Name
Product Synonym Names
Polyclonal Creatine kinase; Anti-Creatine kinase; creatine kinase B chain; CKBB; B-CK; creatine kinase-B; CKB; creatine kinase B-type
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Purity/Purification
Creatine kinase antibody was purified by antigen-affinity chromatography
Concentration
0.54 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
43 kDa
OMIM
123310
3D Structure
ModBase 3D Structure for P06732
Cross-Reactivity
Human, Mouse
Immunogen
Creatine kinase antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 165 and 381 of Creatine kinase
Applications Tested/Suitable for anti-CKM antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-CKM antibody
IF:1:100-1:500
ICC:1:100-1:500
IHC-P: 1:100-1:500
WB: 1:1000-1:10000

Western Blot (WB) of anti-CKM antibody

Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10 % SDS PAGE gel and Creatine kinase antibody at a dilution of 1:10000
Rabbit Anti-CKM Polyclonal Antibody – Cat# MBS835053

Western Blot (WB) of anti-CKM antibody

Western blot analysis of 30 ug of whole cell lysate (A:H1299; B:HeLa S3) using a 12 % SDS PAGE gel and Creatine kinase antibody at a dilution of 1:1000
Rabbit Anti-CKM Polyclonal Antibody, Western Blot (WB) – Cat# MBS835053

Immunofluorescence (IF) of anti-CKM antibody

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Creatine Kinase BB antibody at 1:100 dilution.
Rabbit Anti-CKM Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS835053

Immunohistochemistry (IHC) of anti-CKM antibody

Immunohistochemical staining of paraffin-embedded HSC3 xenograft using Creatine kinase antibody at a dilution of 1:100
Rabbit Anti-CKM Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS835053

Other Notes

Small volumes of anti-CKM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CKM. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
creatine kinase
NCBI Official Synonym Full Names
creatine kinase, muscle
NCBI Official Symbol
CKM
NCBI Official Synonym Symbols
CKMM; M-CK
UniProt Protein Name
Creatine kinase M-type
UniProt Synonym Protein Names
Creatine kinase M chain; M-CK
Protein Family
UniProt Gene Name
CKM
UniProt Synonym Gene Names
CKMM
UniProt Entry Name
KCRM_HUMAN
NCBI Protein Information
creatine kinase M-type

NCBI Summary for CKM

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]
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UniProt Comments for CKM

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Kinase, other; EC 2.7.3.2; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol

Molecular Function: creatine kinase activity; ATP binding

Biological Process: phosphocreatine biosynthetic process; creatine metabolic process; phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CKM antibody

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