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Chitotriosidase (CHIT1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS835061

MBS835061 is an antibody targeting CHIT1. Also known as Chitotriosidase antibody. Host: Rabbit. Affinity purified Rabbit polyclonal Chitotriosidase antibody More details

$1575 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Chitotriosidase (CHIT1), Polyclonal Antibody
Full Product Name
Chitotriosidase antibody
Product Gene Name
Product Synonym Names
Polyclonal Chitotriosidase; Anti-Chitotriosidase; CHI3; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase; chitinase-1; CHIT; FLJ00314; CHIT1; MGC125322; chitotriosidase-1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Purity/Purification
Chitotriosidase antibody was purified by antigen-affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
52 kDa
OMIM
600031
3D Structure
ModBase 3D Structure for Q13231
Cross-Reactivity
Human
Immunogen
Chitotriosidase antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 216 and 463 of Chitotriosidase
Applications Tested/Suitable for anti-CHIT1 antibody
Western Blot (WB)
Application Notes for anti-CHIT1 antibody
WB: 1:500-1:3000

Western Blot (WB) of anti-CHIT1 antibody

Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 10 % SDS PAGE gel and Chitotriosidase antibody at a dilution of 1:1000
Rabbit Anti-CHIT1 Polyclonal Antibody – Cat# MBS835061

Other Notes

Small volumes of anti-CHIT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CHIT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
chitotriosidase
NCBI Official Synonym Full Names
chitinase 1 (chitotriosidase)
NCBI Official Symbol
CHIT1
NCBI Official Synonym Symbols
CHI3; CHIT; CHITD
UniProt Protein Name
Chitotriosidase-1
UniProt Synonym Protein Names
Chitinase-1
Protein Family
UniProt Gene Name
CHIT1
UniProt Entry Name
CHIT1_HUMAN
NCBI Protein Information
chitotriosidase-1

NCBI Summary for CHIT1

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]
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UniProt Comments for CHIT1

CHIT1: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Secreted; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 3.2.1.14; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: extracellular space; lysosome

Molecular Function: endochitinase activity; chitin binding; chitinase activity

Biological Process: polysaccharide catabolic process; response to bacterium; immune response; chitin catabolic process

Disease: Chitotriosidase Deficiency
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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