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BRF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS835275

MBS835275 is an antibody targeting BRF1. Also known as BRF1 antibody. Host: Rabbit. Rabbit polyclonal BRF1 antibody More details

$990 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BRF1, Polyclonal Antibody
Full Product Name
BRF1 antibody
Product Gene Name
anti-BRF1 antibody
Product Synonym Names
Polyclonal BRF1; Anti-BRF1; BRF 1; BRF-1; BRF1
Clonality
Polyclonal
Host
Rabbit
Form/Format
Lyophilized powder. Add 100 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C, following reconstitution, aliquot and store at -20 degree C. Avoid repeated freeze-thaw cycles.
Molecular Weight
73,799 Da
3D Structure
ModBase 3D Structure for Q8CFK2
Cross-Reactivity
Mouse, Rat, Fruit Fly
Immunogen
BRF1 antibody was raised in rabbit using the C terminal of BRF1 as the immunogen

Product Categories/Family for anti-BRF1 antibody

DNA & RNA
Applications Tested/Suitable for anti-BRF1 antibody
Western Blot (WB)
Application Notes for anti-BRF1 antibody
WB: 1.25 ug/ml

Western Blot of anti-BRF1 antibody

Western Blot analysis using BRF1 antibody
BRF1 antibody used at 2.5 ug/ml to detect target protein.
Rabbit Anti-BRF1 Polyclonal Antibody – Cat# MBS835275

Other Notes

Small volumes of anti-BRF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BRF1. It may not necessarily be applicable to this product.

NCBI GI #
34786067
NCBI GeneID
72308
NCBI Accession #
AAH58112
UniProt Primary Accession #
Q8CFK2
UniProt Related Accession #
Q8CFK2
NCBI Official Full Name
Brf1 protein
NCBI Official Synonym Full Names
BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae)
NCBI Official Symbol
Brf1
NCBI Official Synonym Symbols
GTF3B; TAF3C; TAFIII90; TFIIIB90; mTFIIIB90; 2510002F24Rik
UniProt Protein Name
Transcription factor IIIB 90 kDa subunit
UniProt Synonym Protein Names
B-related factor 1; BRF-1
Protein Family
Transcription factor
UniProt Gene Name
Brf1
UniProt Synonym Gene Names
TFIIIB90; mTFIIIB90; BRF-1
UniProt Entry Name
TF3B_MOUSE
NCBI Protein Information
transcription factor IIIB 90 kDa subunit

UniProt Comments for BRF1

GTF3B: General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter. Belongs to the TFIIB family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex

Cellular Component: nucleus; transcription factor TFIIIB complex

Molecular Function: zinc ion binding; TATA-binding protein binding; metal ion binding

Biological Process: transcription initiation from RNA polymerase III promoter; transcription, DNA-dependent; regulation of transcription, DNA-dependent; transcription initiation; positive regulation of transcription from RNA polymerase III promoter; regulation of mRNA stability
Show more

Research Articles on BRF1

1. Brf1 (zfp36l1), an AU-rich element mRNA-binding protein from the Zfp36 protein family, operates downstream of FGF/Erk MAP kinase signaling to regulate pluripotency and cell fate decision making in mouse embryonic stem cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BRF1 antibody

 Reference Product  PubMed Publications
 Tbp antibody  >57 publications with BRF1 and Tbp
 Bdp1 antibody  >27 publications with BRF1 and Bdp1
 Polr2e antibody  >1 publications with BRF1 and Polr2e

Pathways associated with anti-BRF1 antibody

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 RNA Polymerase III Abortive And Retractive Initiation Pathway antibodies  RNA Polymerase III Abortive And Retractive Initiation Pathway Diagram
 RNA Polymerase III Transcription Pathway antibodies  RNA Polymerase III Transcription Pathway Diagram
 RNA Polymerase III Transcription Initiation Pathway antibodies  RNA Polymerase III Transcription Initiation Pathway Diagram
 RNA Polymerase III Transcription Initiation From Type 1 Promoter Pathway antibodies  RNA Polymerase III Transcription Initiation From Type 1 Promoter Pathway Diagram
 RNA Polymerase III Transcription Initiation From Type 2 Promoter Pathway antibodies  RNA Polymerase III Transcription Initiation From Type 2 Promoter Pathway Diagram

Organs/Tissues associated with anti-BRF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >4 publications with BRF1 and Heart
 Muscle Antibodies  >3 publications with BRF1 and Muscle
 Liver Antibodies  >3 publications with BRF1 and Liver
 Bone Antibodies  >2 publications with BRF1 and Bone
 Blood Antibodies  >2 publications with BRF1 and Blood
 Lung Antibodies  >2 publications with BRF1 and Lung
 Pancreas Antibodies  >1 publications with BRF1 and Pancreas
 Uterus Antibodies  >1 publications with BRF1 and Uterus
 Bone Marrow Antibodies  >1 publications with BRF1 and Bone Marrow
 Embryonic Tissue Antibodies  >1 publications with BRF1 and Embryonic Tissue
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