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Cytokeratin 20 (KRT20) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS835335

MBS835335 is an antibody targeting KRT20. Also known as Cytokeratin 20 antibody. Host: Rabbit. Affinity purified Rabbit polyclonal Cytokeratin 20 antibody More details

$1565 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 20 (KRT20), Polyclonal Antibody
Full Product Name
Cytokeratin 20 antibody
Product Gene Name
Product Synonym Names
Polyclonal Cytokeratin 20; Anti-Cytokeratin 20; Cytokeratin 20; MGC35423; cytokeratin-20; K20; KRT21; CK-20; KRT20; CK20; Cytokeratin -20; keratin-20; CD20
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Purity/Purification
Cytokeratin 20 antibody was purified by antigen-affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
49 kDa
OMIM
608218
3D Structure
ModBase 3D Structure for P35900
Cross-Reactivity
Human
Applications Tested/Suitable for anti-KRT20 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-KRT20 antibody
IF:1:100-1:200
ICC:1:100-1:200
IHC-P: 1:100-1:500
WB: 1:500-1:3000

Immunofluorescence (IF) of anti-KRT20 antibody

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20 antibody at 1:200 dilution.
Rabbit Anti-KRT20 Polyclonal Antibody – Cat# MBS835335

Western Blot (WB) of anti-KRT20 antibody

Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 10 % SDS PAGE gel and Cytokeratin 20 antibody at a dilution of 1:3000
Rabbit Anti-KRT20 Polyclonal Antibody, Western Blot (WB) – Cat# MBS835335

Immunohistochemistry (IHC) of anti-KRT20 antibody

Immunohistochemical staining of paraffin-embedded HT29 xenograft using Cytokeratin 20 antibody at a dilution of 1:100
Rabbit Anti-KRT20 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS835335

Other Notes

Small volumes of anti-KRT20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT20. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
cytokeratin 20
UniProt Protein Name
Keratin, type I cytoskeletal 20
UniProt Synonym Protein Names
Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT
Protein Family
UniProt Gene Name
KRT20
UniProt Synonym Gene Names
CK-20; K20
UniProt Entry Name
K1C20_HUMAN

UniProt Comments for KRT20

K20: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; intermediate filament

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: regulation of protein secretion; apoptosis; cellular response to stress; intermediate filament organization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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