• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

EGF Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS836477

MBS836477 is an active protein targeting EGF. Also known as EGF protein. Host: Human. Purified recombinant Human EGF protein More details

$325 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EGF, Active Protein
Full Product Name
EGF protein
Product Gene Name
Product Synonym Names
Epidermal growth factor protein; Urogastrone protein; epidermal growth factor (beta-urogastrone); EGF protein; Pro-epidermal growth factor protein
Sequence
MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR
Host
Human
Form/Format
Supplied as a liquid in PBS, pH 7.4.
Purity/Purification
> 95% pure
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
6.3 kDa (54aa), confirmed by MALDI-TOF.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Protein Type
Recombinant
Bioactivity
The ED50 for this effect is < 0.89 ng/ml. Measured in a cell proliferation assay using mouse Balb3T3 cell.
Expression System
E Coli

Product Categories/Family for EGF active protein

Applications Tested/Suitable for EGF active protein
SDS-PAGE
Application Notes for EGF active protein
Cell line: Balb-3T3 (Mus musculus (mouse)) Maintenance Condition: RPMI 1640 containing 10% FBS Assay medium: serum free RPMI 1640 Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Serum Free Starvation: 24hr with RPMI1640 Incubation time: 24 hr (after sample treatment) Concentration range: 1 pg/ml - 1 ug/ml Detection method: BrdU assay

SDS-PAGE of EGF active protein

Coomassie Blue stained SDS-PAGE of EGF protein
Figure annotation denotes ug of protein loaded and % gel used.
EGF Active Protein – Cat# MBS836477

Other Notes

Small volumes of EGF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EGF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
epidermal growth factor, partial
UniProt Protein Name
Epidermal growth factor
UniProt Synonym Protein Names
Epidermal growth factor
UniProt Gene Name
epidermal growth factor/ EGF
UniProt Entry Name
Q28867_HORSE

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000