Figure annotation denotes ug of protein loaded and % gel used.

PPIF Active Protein

Name: PPIF Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS836606
Price: 540 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS836606

MBS836606 is an active protein targeting PPIF. Also known as PPIF protein (His tag). Host: Human. Purified recombinant Human PPIF protein More details

$540 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PPIF, Active Protein

Full Product Name

PPIF protein (His tag)

Product Gene Name

PPIF active protein

Product Synonym Names

Peptidylprolyl isomerase F Cyclophilin 3 protein; Peptidylprolyl isomerase F (cyclophilin F) protein; Rotamase.; Peptidyl prolyl cis trans isomerase protein; Cyclophilin F protein; Cyclophilin D protein; CYP3 protein; Peptidylprolyl isomerase F protein; PPIase protein; mitochondral protein; hCyP3 protein; Cyp D protein; PPIF protein; CypD protein; Cyp-D protein

Sequence

30-207 amino acids: MGSSHHHHHH SSGLVPRGSH CSKGSGDPSS SSSSGNPLVY LDVDANGKPL GRVVLELKAD VVPKTAENFR ALCTGEKGFG YKGSTFHRVI PSFMCQAGDF TNHNGTGGKS IYGSRFPDEN FTLKHVGPGV LSMANAGPNT NGSQFFICTI KTDWLDGKHV VFGHVKEGMD VVKKIESFGS KSGRTSKKIV ITDCGQLS

Host

Human

Form/Format

Supplied as a liquid in 20mM Tris, pH 7.5, containing 1mM DTT, and 10% glycerol.

Purity/Purification

> 95% pure

Concentration

1 mg/ml (lot specific)

Preparation and Storage

Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Molecular Weight

21 kDa (198aa), confirmed by MALDI-TOF.

OMIM

604486

3D Structure

ModBase 3D Structure for P30405

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Protein Type

Recombinant

Bioactivity

Specific activity is > 120 nM/min/ug, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 degree C in Tris-Hcl pH 8.0 using chymotrypsin.

Endotoxin Levels

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Expression System

E Coli

Tag/Conjugate

His tag

Product Categories/Family for PPIF active protein

Proteases; Inhibitors; & Enzymes; Conjugated Proteins

Applications Tested/Suitable for PPIF active protein

SDS-PAGE

Application Notes for PPIF active protein

1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant Cyclophilin F protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes

Other Notes

Small volumes of PPIF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPIF. It may not necessarily be applicable to this product.

NCBI GI #

5031987

NCBI GeneID

10105

NCBI Accession #

NP_005720.1

UniProt Primary Accession #

P30405

UniProt Secondary Accession #

Q2YDB7; Q5W131

UniProt Related Accession #

P30405

GenBank Nucleotide #

NM_005729.3

NCBI Official Full Name

peptidyl-prolyl cis-trans isomerase F, mitochondrial

NCBI Official Synonym Full Names

peptidylprolyl isomerase F

NCBI Official Symbol

PPIF

NCBI Official Synonym Symbols

CYP3; CypD; CyP-M; Cyp-D

UniProt Protein Name

Peptidyl-prolyl cis-trans isomerase F, mitochondrial

UniProt Synonym Protein Names

Cyclophilin D; CyP-D; CypD; Cyclophilin F; Mitochondrial cyclophilin; CyP-M; Rotamase F

Protein Family

Peptidyl-prolyl cis-trans isomerase

UniProt Gene Name

PPIF

UniProt Synonym Gene Names

CYP3; PPIase F; CyP-D; CypD; CyP-M

UniProt Entry Name

PPIF_HUMAN

NCBI Protein Information

peptidyl-prolyl cis-trans isomerase F, mitochondrial

NCBI Summary for PPIF

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]

UniProt Comments for PPIF

PPIF: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family.

Protein type: EC 5.2.1.8; Isomerase; Mitochondrial

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex

Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; cyclosporin A binding

Biological Process: apoptotic mitochondrial changes; protein peptidyl-prolyl isomerization; protein folding; regulation of mitochondrial membrane permeability; negative regulation of ATPase activity; negative regulation of apoptosis

Research Articles on PPIF

1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PPIF active protein

Products by Pathway	Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Huntington's Disease Pathway antibodies	Huntington's Disease Pathway Diagram
Huntington's Disease Pathway antibodies	Huntington's Disease Pathway Diagram
Parkinson's Disease Pathway antibodies	Parkinson's Disease Pathway Diagram
Toxoplasmosis Pathway antibodies	Toxoplasmosis Pathway Diagram
Toxoplasmosis Pathway antibodies	Toxoplasmosis Pathway Diagram
CGMP-PKG Signaling Pathway antibodies	CGMP-PKG Signaling Pathway Diagram
CGMP-PKG Signaling Pathway antibodies	CGMP-PKG Signaling Pathway Diagram

Diseases associated with PPIF active protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>8 publications with PPIF and Necrosis
Cardiovascular Diseases Antibodies	>5 publications with PPIF and Cardiovascular Diseases
Disease Models, Animal Antibodies	>5 publications with PPIF and Disease Models, Animal
Liver Diseases Antibodies	>2 publications with PPIF and Liver Diseases
Drug-Related Side Effects and Adverse Reactions Antibodies	>2 publications with PPIF and Drug-Related Side Effects and Adverse Reactions
Drug-Induced Liver Injury Antibodies	>2 publications with PPIF and Drug-Induced Liver Injury
Poisoning Antibodies	>2 publications with PPIF and Poisoning
Nervous System Diseases Antibodies	>2 publications with PPIF and Nervous System Diseases
Hypertrophy Antibodies	>2 publications with PPIF and Hypertrophy
Fibrosis Antibodies	>1 publications with PPIF and Fibrosis

Organs/Tissues associated with PPIF active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>9 publications with PPIF and Brain
Heart Antibodies	>5 publications with PPIF and Heart
Liver Antibodies	>5 publications with PPIF and Liver
Muscle Antibodies	>4 publications with PPIF and Muscle
Blood Antibodies	>3 publications with PPIF and Blood
Kidney Antibodies	>2 publications with PPIF and Kidney
Embryonic Tissue Antibodies	>1 publications with PPIF and Embryonic Tissue
Intestine Antibodies	>1 publications with PPIF and Intestine
Lung Antibodies	>1 publications with PPIF and Lung
Pancreas Antibodies	>1 publications with PPIF and Pancreas