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DC8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS837410

MBS837410 is an antibody targeting DC8. Also known as DC8 antibody. Host: Rabbit. Affinity purified Rabbit polyclonal DC8 antibody More details

$1565 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DC8, Polyclonal Antibody
Full Product Name
DC8 antibody
Product Gene Name
Product Synonym Names
Polyclonal DC8; Anti-DC8; NSL1; DKFZp566O1646; MIS14; DC8; C1orf48; DC 8; DC-8; kinetochore-associated protein NSL1 homolog
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Purity/Purification
DC8 antibody was purified by antigen-affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
32 kDa
OMIM
609174
3D Structure
ModBase 3D Structure for Q96IY1
Cross-Reactivity
Human
Immunogen
DC8 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 275 of DC8
Applications Tested/Suitable for anti-DC8 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-DC8 antibody
IF:1:100-1:200
WB: 1:500-1:3000

Immunofluorescence (IF) of anti-DC8 antibody

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DC8 antibody at 1:200 dilution.
Rabbit Anti-DC8 Polyclonal Antibody – Cat# MBS837410

Western Blot (WB) of anti-DC8 antibody

Western blot analysis of 30 ug of whole cell lysate (A:Hep G2) using a 12 % SDS PAGE gel and DC8 antibody at a dilution of 1:1000
Rabbit Anti-DC8 Polyclonal Antibody, Western Blot (WB) – Cat# MBS837410

Other Notes

Small volumes of anti-DC8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DC8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
DC8
NCBI Official Synonym Full Names
NSL1, MIS12 kinetochore complex component
NCBI Official Symbol
NSL1
NCBI Official Synonym Symbols
DC8; MIS14; C1orf48
UniProt Protein Name
Kinetochore-associated protein NSL1 homolog
UniProt Gene Name
NSL1
UniProt Synonym Gene Names
C1orf48
UniProt Entry Name
NSL1_HUMAN
NCBI Protein Information
kinetochore-associated protein NSL1 homolog

NCBI Summary for DC8

This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. There is a pseudogene of the 3' UTR region of this gene on chromosome X. [provided by RefSeq, Jul 2014]
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UniProt Comments for DC8

NSL1: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: cytosol; nucleus; MIS12/MIND type complex

Molecular Function: protein binding

Biological Process: mitosis; cell division; mitotic cell cycle; chromosome segregation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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