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CDCA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS837530

MBS837530 is an antibody targeting CDCA1. Also known as CDCA1 antibody. Host: Rabbit. Affinity purified Rabbit polyclonal CDCA1 antibody More details

$1575 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CDCA1, Polyclonal Antibody
Full Product Name
CDCA1 antibody
Product Gene Name
Product Synonym Names
Polyclonal CDCA1; Anti-CDCA1; CDCA 1; hNuf2R; cell division cycle associated 1; NUF2; kinetochore protein Nuf2; cell division cycle-associated protein 1; CDCA1; hsNuf2; NUF2R; cancer/testis antigen 106; CT106; CDCA-1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Purity/Purification
CDCA1 antibody was purified by antigen-affinity chromatography
Concentration
0.35 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
54,304 Da
OMIM
611772
3D Structure
ModBase 3D Structure for Q9BZD4
Cross-Reactivity
Human
Applications Tested/Suitable for anti-CDCA1 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-CDCA1 antibody
IF:1:100-1:1000
ICC:1:100-1:1000
WB: 1:500-1:3000

Immunofluorescence (IF) of anti-CDCA1 antibody

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using CDCA1 antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Rabbit Anti-CDCA1 Polyclonal Antibody – Cat# MBS837530

Western Blot (WB) of anti-CDCA1 antibody

Western blot analysis of 30 ug of whole cell lysate (A: HeLa; B: HeLa nucleus) using a 10 % SDS PAGE gel and CDCA1 antibody at a dilution of 1:5000
Rabbit Anti-CDCA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS837530

Other Notes

Small volumes of anti-CDCA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDCA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
cell division cycle associated 1, isoform CRA_a
NCBI Official Synonym Full Names
NUF2, NDC80 kinetochore complex component
NCBI Official Symbol
NUF2
NCBI Official Synonym Symbols
CDCA1; CT106; NUF2R
UniProt Protein Name
Kinetochore protein Nuf2
UniProt Synonym Protein Names
Cell division cycle-associated protein 1
UniProt Gene Name
NUF2
UniProt Synonym Gene Names
CDCA1; NUF2R; hNuf2; hNuf2R; hsNuf2
UniProt Entry Name
NUF2_HUMAN
NCBI Protein Information
kinetochore protein Nuf2

NCBI Summary for CDCA1

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for CDCA1

NUF2: Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. Belongs to the NUF2 family.

Protein type: Cancer Testis Antigen (CTA); DNA replication

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: membrane; cytosol; nucleus; chromosome, pericentric region

Molecular Function: protein binding

Biological Process: mitosis; cell division; mitotic cell cycle; chromosome segregation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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