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ERK8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS838959

MBS838959 is an antibody targeting ERK8. Also known as ERK8 antibody (Thr175+Tyr177). Host: Rabbit. Rabbit polyclonal ERK8 antibody (Thr175+Tyr177) More details

$695 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ERK8, Polyclonal Antibody
Full Product Name
ERK8 antibody (Thr175+Tyr177)
Product Gene Name
Product Synonym Names
Polyclonal ERK8; Anti-ERK8; Mitogen-Activated Protein Kinase 15; Extracellular Signal Regulated Kinase 8; ERK 7; ERK-7; MAP Kinase 15; ERK 8; ERK-8
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied in PBS buffer (without Mg2+and Ca2+), pH 7.4, 150mM NaCl with 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (lot specific)
Specificity
ERK8 antibody detects endogenous levels of ERK8 only when phosphorylated at threonine 175 and tyrosine 177
Preparation and Storage
Store at -20 degree C for 1 year, store at 2-8 degree C for up to 1 month
Molecular Weight
31,112 Da
Cross-Reactivity
Mouse
Immunogen
ERK8 antibody was raised in Rabbit using a synthetic phosphopeptide derived from human ERK8 around the phosphorylation site of threonine 175 and tyrosine 177 (A-V-TP-E-YP-V-A) as the immunogen
Applications Tested/Suitable for anti-ERK8 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-ERK8 antibody
WB: 1:500-1:1000
ELISA: 1:5000

Western Blot (WB) of anti-ERK8 antibody

Western blot analysis of extracts from Jurkat cells, using ERK8 (Phospho-Thr175+Tyr177) antibody.
Rabbit Anti-ERK8 Polyclonal Antibody – Cat# MBS838959

Other Notes

Small volumes of anti-ERK8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ERK8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Secondary Accession #
NCBI Official Full Name
extracellular regulated kinase 8 delta
UniProt Protein Name
Mitogen-activated protein kinase 15
UniProt Synonym Protein Names
Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8
UniProt Gene Name
MAPK15
UniProt Synonym Gene Names
ERK7; ERK8; MAP kinase 15; MAPK 15; ERK-7; ERK-8
UniProt Entry Name
MK15_HUMAN

UniProt Comments for ERK8

ERK7: In vitro, phosphorylates MBP. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.24; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; MAPK family; Erk7 subfamily

Chromosomal Location of Human Ortholog: -

Cellular Component: extracellular region; intracellular; nucleus

Molecular Function: MAP kinase activity; SH3 domain binding; ATP binding

Biological Process: positive regulation of protein ubiquitination; negative regulation of DNA replication; protein amino acid autophosphorylation; MAPKKK cascade; positive regulation of protein catabolic process; positive regulation of protein amino acid phosphorylation; negative regulation of transcription from RNA polymerase II promoter; response to estradiol stimulus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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