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Nucleolin (NCL) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS839516

MBS839516 is an antibody targeting NCL. Also known as Nucleolin antibody. Host: Rabbit. More details

$980 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nucleolin (NCL), Polyclonal Antibody
Full Product Name
Nucleolin antibody
Product Gene Name
Product Synonym Names
Polyclonal Nucleolin; Anti-Nucleolin; NCL
Clonality
Polyclonal
Host
Rabbit
Form/Format
Purified antibody supplied as a liquid in 1X PBS buffer with 0.09% NaN3 , and 2% Sucrose.
Purity/Purification
Total IgG Protein A purified
Concentration
1 mg/ml (lot specific)
Specificity
Nucleolin antibody was raised against the C terminal of NCL
Preparation and Storage
Store at 4°C for short term storage. For long term Storage, store at -20°C. Avoid repeated freeze-thaws.
Molecular Weight
78 kDa (MW of target protein)
OMIM
164035
Cross-Reactivity
Human, Mouse, Rat, Cow, Dog, Guinea Pig , Horse, Rabbit
Immunogen
Nucleolin antibody was raised using the C terminal of NCL corresponding to a region with amino acids GGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE
Applications Tested/Suitable for anti-NCL antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-NCL antibody
WB: 1.25 ug/ml
IHC: 4-8 ug/ml

Western Blot (WB) of anti-NCL antibody

Nucleolin antibody (MBS839516) used at 1.25 ug/ml to detect target protein.
Rabbit Anti-NCL Polyclonal Antibody – Cat# MBS839516

Immunohistochemistry (IHC) of anti-NCL antibody

Nucleolin antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Epithelial cells of renal tubule (arrows) in Human Kidney. Magnification is at 400X.
Rabbit Anti-NCL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS839516

Other Notes

Small volumes of anti-NCL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NCL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nucleolin
NCBI Official Synonym Full Names
nucleolin
NCBI Official Symbol
NCL
NCBI Official Synonym Symbols
C23
UniProt Protein Name
Nucleolin
UniProt Synonym Protein Names
Protein C23
Protein Family
UniProt Gene Name
NCL
UniProt Entry Name
NUCL_HUMAN
NCBI Protein Information
nucleolin

NCBI Summary for NCL

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
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UniProt Comments for NCL

NCL: the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-RNA transcription and ribosome assembly.

Protein type: Nuclear receptor co-regulator; RNA-binding; Nucleolus; DNA-binding; Helicase

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: nucleoplasm; membrane; nucleolus; cell cortex; nucleus; ribonucleoprotein complex

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; RNA binding; nucleotide binding; telomeric DNA binding

Biological Process: angiogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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