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MAP1LC3A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9200215

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded… More details

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Product Overview

Online Datasheet in PDF
Product Name
LC3 (MAP1LC3A), Monoclonal Antibody
Full Product Name
LC3 Antibody (APG8)
Product Gene Name
anti-MAP1LC3A antibody
Product Synonym Names
Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha; MAP1LC3A
Clonality
Monoclonal
Isotype
Mouse IgG1 k
Host
Mouse
Species Reactivity
Human, mouse, rat
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purity/Purification
This antibody is purified through a protein G column, followed by dialysis against PBS.
Specificity
This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
LC3 (APG8A) peptide (MBS9223036) is used for blocking the activity of LC3 Clone #166AT1234 antibody (MBS9200215)
OMIM
601242
3D Structure
ModBase 3D Structure for Q9H492

Product Categories/Family for anti-MAP1LC3A antibody

Signal Transduction; Neuroscience; Microbiology; Metabolism; Cell Biology; Cardiovascular; Cancer; Autophagy
Applications Tested/Suitable for anti-MAP1LC3A antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-MAP1LC3A antibody
WB~~1:8000

Western Blot (WB) of anti-MAP1LC3A antibody

Anti-MAP1LC3A Antibody at 1:5000 dilution + recombinant protein Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Mouse Anti-MAP1LC3A Monoclonal Antibody – Cat# MBS9200215

Western Blot (WB) of anti-MAP1LC3A antibody

All lanes : Anti-MAP1LC3A Antibody at dilution
Lane 1: Mouse brain whole cell lysate
Lane 2:Rat brain whole cell lysate
Lane 3: Human brain whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 14 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Mouse Anti-MAP1LC3A Monoclonal Antibody, Western Blot (WB) – Cat# MBS9200215

Immunofluorescence (IF) of anti-MAP1LC3A antibody

Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8). U251 cells(right) were treated with Chloroquine (50 uM,16h). MBS9200215 was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Mouse Anti-MAP1LC3A Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS9200215

Immunofluorescence (IF) of anti-MAP1LC3A antibody

Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8). U251 cells(right) were treated with Chloroquine (50 uM,16h). MBS9200215 was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Mouse Anti-MAP1LC3A Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS9200215

Immunofluorescence (IF) of anti-MAP1LC3A antibody

Fluorescent image of U251 cells stained with MBS9200215 LC3 (APG8) antibody.U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9200215 LC3 (APG8) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Mouse Anti-MAP1LC3A Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS9200215

Western Blot (WB) of anti-MAP1LC3A antibody

Western blot analysis of anti-LC3 Mab at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).
Mouse Anti-MAP1LC3A Monoclonal Antibody, Western Blot (WB) – Cat# MBS9200215

Western Blot (WB) of anti-MAP1LC3A antibody

Western blot analysis of anti-LC3 Mab Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.
Mouse Anti-MAP1LC3A Monoclonal Antibody, Western Blot (WB) – Cat# MBS9200215

Immunohistochemistry (IHC) of anti-MAP1LC3A antibody

10X(lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Primary antibody used is. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.
Mouse Anti-MAP1LC3A Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9200215

Product References and Citations for anti-MAP1LC3A antibody

References for protein:
1.Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation. Gao C, et al. Nat Cell Biol, 2010 Aug. PMID 20639871.
2.The prolyl isomerase Pin1 induces LC-3 expression and mediates tamoxifen resistance in breast cancer. Namgoong GM, et al. J Biol Chem, 2010 Jul 30. PMID 20479004.
3.Protein kinase C inhibits autophagy and phosphorylates LC3. Jiang H, et al. Biochem Biophys Res Commun, 2010 May 14. PMID 20398630.
4. Processing of autophagic protein LC3 by the 20S proteasome. Gao Z, et al. Autophagy, 2010 Jan. PMID 20061800.
5.Defective autophagy associated with LC3 puncta in epothilone-resistant cancer cells. Shen S, et al. Cell Cycle, 2010 Jan 15. PMID 20023420.
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].

Other Notes

Small volumes of anti-MAP1LC3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAP1LC3A purchase

MBS9200215 is an antibody targeting MAP1LC3A. Also known as LC3 Antibody (APG8). Reactive in Human, mouse, rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP1LC3A. It may not necessarily be applicable to this product.

NCBI GI #
14210522
NCBI GeneID
84557
NCBI Accession #
NP_115903.1
NCBI Related Accession #
NP_852610.1
UniProt Primary Accession #
Q9H492
UniProt Secondary Accession #
Q9BXW5; E1P5P4; E1P5P5
UniProt Related Accession #
Q9H492
GenBank Nucleotide #
NM_032514.3
NCBI Official Full Name
microtubule-associated proteins 1A/1B light chain 3A isoform a
NCBI Official Synonym Full Names
microtubule-associated protein 1 light chain 3 alpha
NCBI Official Symbol
MAP1LC3A
NCBI Official Synonym Symbols
LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
UniProt Protein Name
Microtubule-associated proteins 1A/1B light chain 3A
UniProt Synonym Protein Names
Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha
Protein Family
Microtubule-associated protein
UniProt Gene Name
MAP1LC3A
UniProt Synonym Gene Names
MAP1A/MAP1B LC3 A
UniProt Entry Name
MLP3A_HUMAN
NCBI Protein Information
microtubule-associated proteins 1A/1B light chain 3A

NCBI Summary for MAP1LC3A

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]
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UniProt Comments for MAP1LC3A

LC3A: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding; Ubiquitin-like modifier; Vesicle; Autophagy

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: microtubule; extrinsic to membrane; late endosome; autophagic vacuole; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; phosphatidylethanolamine binding; microtubule binding; GABA receptor binding; phospholipid binding

Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation
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Research Articles on MAP1LC3A

1. A significant association of LC3 expression with disease progression including a large tumor size, higher stage, and worse prognosis of hepatocellular carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAP1LC3A antibody

 Reference Product  PubMed Publications
 SQSTM1 antibody  >25 publications with MAP1LC3A and SQSTM1
 ATG5 antibody  >20 publications with MAP1LC3A and ATG5
 ATG7 antibody  >13 publications with MAP1LC3A and ATG7
 ULK1 antibody  >6 publications with MAP1LC3A and ULK1
 ATG4B antibody  >6 publications with MAP1LC3A and ATG4B
 ATG3 antibody  >2 publications with MAP1LC3A and ATG3
 ATG13 antibody  >1 publications with MAP1LC3A and ATG13
 ATG4A antibody  >1 publications with MAP1LC3A and ATG4A
 NBR1 antibody  >1 publications with MAP1LC3A and NBR1

Pathways associated with anti-MAP1LC3A antibody

 Products by Pathway  Pathway Diagram
 , Pathway antibodies  , Pathway Diagram

Diseases associated with anti-MAP1LC3A antibody

 Disease Name  Pubmed Publications
 Death Antibodies  >51 publications with MAP1LC3A and Death
 Lung Diseases Antibodies  >14 publications with MAP1LC3A and Lung Diseases
 Nervous System Diseases Antibodies  >13 publications with MAP1LC3A and Nervous System Diseases
 Disease Models, Animal Antibodies  >6 publications with MAP1LC3A and Disease Models, Animal
 Liver Neoplasms Antibodies  >5 publications with MAP1LC3A and Liver Neoplasms
 Neoplasm Metastasis Antibodies  >5 publications with MAP1LC3A and Neoplasm Metastasis
 Inflammation Antibodies  >4 publications with MAP1LC3A and Inflammation
 Liver Diseases Antibodies  >3 publications with MAP1LC3A and Liver Diseases
 Cardiovascular Diseases Antibodies  >3 publications with MAP1LC3A and Cardiovascular Diseases
 Kidney Diseases Antibodies  >3 publications with MAP1LC3A and Kidney Diseases

Organs/Tissues associated with anti-MAP1LC3A antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >17 publications with MAP1LC3A and Lung
 Brain Antibodies  >13 publications with MAP1LC3A and Brain
 Liver Antibodies  >9 publications with MAP1LC3A and Liver
 Muscle Antibodies  >9 publications with MAP1LC3A and Muscle
 Kidney Antibodies  >5 publications with MAP1LC3A and Kidney
 Intestine Antibodies  >4 publications with MAP1LC3A and Intestine
 Eye Antibodies  >4 publications with MAP1LC3A and Eye
 Nerve Antibodies  >3 publications with MAP1LC3A and Nerve
 Skin Antibodies  >3 publications with MAP1LC3A and Skin
 Prostate Antibodies  >3 publications with MAP1LC3A and Prostate
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