DNA Methyltransferase 3a (DNMT3A) Antibody – Mouse Monoclonal

This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]

DNMT3A: a DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Prefers CpG methylation to CpA, CpT, and CpC methylation. Methylates CpG sites at a rate much slower than DNMT1, but greater than DNMT3b. Methylation is coordinated with methylation of histones. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1 and DNMT1. Can co-localize with heterochromatin protein (HP1 ) and methyl-CpG binding protein (MeCBP). Associates with HDAC1 through its ADD-type zinc-finger. Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Protein type: Methyltransferase; Amino Acid Metabolism - cysteine and methionine; Methyltransferase, DNA; EC 2.1.1.37

Cellular Component: nucleoplasm; XY body; heterochromatin; euchromatin; nuclear matrix; cytoplasm; nuclear heterochromatin; nucleus; chromosome, pericentric region

Molecular Function: transferase activity; DNA (cytosine-5-)-methyltransferase activity; methyltransferase activity; identical protein binding; protein binding; DNA binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; unmethylated CpG binding; metal ion binding; chromatin binding

Biological Process: methylation; genetic imprinting; maintenance of DNA methylation; transcription, DNA-dependent; DNA methylation during gametogenesis; S-adenosylhomocysteine metabolic process; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; cytosine methylation within a CG sequence; regulation of transcription, DNA-dependent; methylation-dependent chromatin silencing; DNA methylation; S-adenosylmethioninamine metabolic process; spermatogenesis; DNA methylation during embryonic development; DNA methylation on cytosine