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HSP47 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS840245

Heat shock proteins (HSPs) are ubiquitously expressed in all organisms. HSP47 is a molecule expressed constitutively in cells that synthesizes collagen. HSP47 is a procollagen-specific molecular chaperon that resides in the endoplasmic reticulum of… More details

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Product Overview

Product Name
Hsp47, Polyclonal Antibody
Full Product Name
Hsp47 Antibody
Product Gene Name
Product Synonym Names
SERPINH1; AsTP3; CBP1; CBP2; HSP47; PIG14; RA-A47; PPROM; colligen; colligin-2; SERPINH2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Chick
Form/Format
0.2 mg/ml Protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Specificity
The antibody recognizes 47 kDa Hsp47 of human, mouse, rat, and chick origins. Mouse small intestine tissue lysate can be used as a positive control.
Preparation and Storage
Store at -20°C. For long term storage, aliquot and freeze at –70°C. Avoid repeated freeze/defrost cycles.
3D Structure
ModBase 3D Structure for P19324
Immunogen
Synthetic peptide surrounding amino acid 407 of human HSP47 (ID# BV-341).
Applications Tested/Suitable for anti-Hsp47 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-Hsp47 antibody
Recommended Dilution:
Western blotting (0.5-4 ug/ml)
Immunohistochemistry (2.5 ug/ml)
Immunoprecipitation (10-20 ug/ml)

The optimal concentrations should be determined individually.
Blocking peptide (Cat. # MBS843708) is also available.

Western Blot (WB) of anti-Hsp47 antibody

Rabbit Anti-HSP47 Polyclonal Antibody – Cat# MBS840245

Other Notes

Small volumes of anti-Hsp47 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hsp47 purchase

MBS840245 is an antibody targeting Hsp47. Also known as Hsp47 Antibody. Reactive in Human, Mouse, Rat, Chick. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Hsp47. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serpin H1
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade H, member 1
NCBI Official Symbol
Serpinh1
NCBI Official Synonym Symbols
J6; Cbp1; Cbp2; gp46; Hsp47; BERF-1; Serpinh2
UniProt Protein Name
Serpin H1
UniProt Synonym Protein Names
47 kDa heat shock protein; Collagen-binding protein; Colligin; Serine protease inhibitor J6
UniProt Gene Name
Serpinh1
UniProt Synonym Gene Names
Cbp1; Hsp47; Colligin
UniProt Entry Name
SERPH_MOUSE
NCBI Protein Information
serpin H1

UniProt Comments for Hsp47

SERPINH1: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. Defects in SERPINH1 are the cause of osteogenesis imperfecta type 10 (OI10). A connective tissue disorder characterized by bone fragility, low bone mass, bowing of limbs due to multiple fractures, short limb dwarfism and blue sclerae. Belongs to the serpin family.

Cellular Component: extracellular space; endoplasmic reticulum; cytoplasm; ER-Golgi intermediate compartment

Molecular Function: serine-type endopeptidase inhibitor activity; collagen binding; protein binding; unfolded protein binding

Biological Process: collagen fibril organization; protein maturation; collagen biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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