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DFF45 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS840263

Background: Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as many other types of diseases. Recently, a human DNA fragmentation factor (DFF45)… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
DFF45, Polyclonal Antibody
Full Product Name
DFF45 Antibody
Product Gene Name
Product Synonym Names
DFFA; DFF-45; ICAD; DFF1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
100 ug (0.2 mg/ml) immunoaffinity purified rabbit DFF45 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Appearance: Colorless liquid
Purity/Purification
Affinity purified
Concentration
0.2 mg/ml (lot specific)
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
29,183 Da
Immunogen
Synthetic peptide mapping to the N-terminus of mouse DFF45
Handling
The antibody solution should be gently mixed before use.
Applications Tested/Suitable for anti-DFF45 antibody
Western Blot (WB)
Application Notes for anti-DFF45 antibody
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects the 45 kDa and 28 kDa proteins, corresponding to the apparent molecular weight of DFF45/ICADL, and ICADS in immunoblots.

Other Notes

Small volumes of anti-DFF45 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DFF45 purchase

MBS840263 is an antibody targeting DFF45. Also known as DFF45 Antibody. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DFF45. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
DNA fragmentation factor, alpha subunit
NCBI Official Synonym Full Names
DNA fragmentation factor, alpha subunit
NCBI Official Symbol
Dffa
NCBI Official Synonym Symbols
ICAD; DFF35; Dff45; ICAD-L; ICAD-S; A330085O09Rik
UniProt Protein Name
DNA fragmentation factor subunit alpha
UniProt Synonym Protein Names
DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD
UniProt Gene Name
Dffa
UniProt Synonym Gene Names
Icad; DFF-45; ICAD
UniProt Entry Name
DFFA_MOUSE
NCBI Protein Information
DNA fragmentation factor subunit alpha

UniProt Comments for DFF45

DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase

Cellular Component: nucleoplasm; cytoplasm; nuclear chromatin; intracellular; nucleus; cytosol

Molecular Function: protein binding

Biological Process: positive regulation of apoptosis; apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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